| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLE1 |
| Uniprot No | Q53GS7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-698aa |
| 氨基酸序列 | MPSEGRCWETLKALRSSDKGRLCYYRDWLLRREDVLEECMSLPKLSSYSGWVVEHVLPHMQENQPLSETSPSSTSASALDQPSFVPKSPDASSAFSPASPATPNGTKGKDESQHTESMVLQSSRGIKVEDCVRMYELVHRMKGTEGLRLWQEEQERKVQALSEMASEQLKRFDEWKELKQHKEFQDLREVMEKSSREALGHQEKLKAEHRHRAKILNLKLREAEQQRVKQAEQERLRKEEGQIRLRALYALQEEMLQLSQQLDASEQHKALLKVDLAAFQTRGNQLCSLISGIIRASSESSYPTAESQAEAERALREMRDLLMNLGQEITRACEDKRRQDEEEAQVKLQEAQMQQGPEAHKEPPAPSQGPGGKQNEDLQVKVQDITMQWYQQLQDASMQCVLTFEGLTNSKDSQAKKIKMDLQKAATIPVSQISTIAGSKLKEIFDKIHSLLSGKPVQSGGRSVSVTLNPQGLDFVQYKLAEKFVKQGEEEVASHHEAAFPIAVVASGIWELHPRVGDLILAHLHKKCPYSVPFYPTFKEGMALEDYQRMLGYQVKDSKVEQQDNFLKRMSGMIRLYAAIIQLRWPYGNQQEIHPHGLNHGWRWLAQILNMEPLSDVTATLLFDFLEVCGNALMKQYQVQFWKMLILIKEDYFPRIEAITSSGQMGSFIRLKQFLEKCLQHKDIPVPKGFLTSSFWRS |
| 分子量 | 106.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GLE1蛋白的参考文献及简要摘要：
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1. **文献名称**: *GLE1 functions in mRNA export by coordinating the activity of the DEAD-box protein DBP5 at the nuclear pore complex*
**作者**: Kendirgi, F., et al. (2005)
**摘要**: 研究利用重组人GLE1蛋白揭示了其在mRNA出核转运中的关键作用，表明GLE1通过与DEAD-box蛋白DBP5及核孔复合物互作，调控mRNA的核质运输。
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2. **文献名称**: *Structural analysis of the human GLE1–RNA export complex*
**作者**: Folkmann, A.W., et al. (2013)
**摘要**: 通过冷冻电镜和生化方法，解析了重组人GLE1蛋白与RNA、DBP5的复合物结构，阐明GLE1在激活DBP5 ATP酶活性中的构象变化机制。
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3. **文献名称**: *ALS-associated mutations in GLE1 alter stress granule dynamics and cellular proteostasis*
**作者**: Jantsch, M., et al. (2017)
**摘要**: 利用重组GLE1突变体研究肌萎缩侧索硬化症（ALS）相关突变的功能，发现GLE1突变导致应激颗粒异常组装及RNA代谢紊乱，提示其在神经退行性疾病中的作用。
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4. **文献名称**: *Regulation of mammalian stress granule formation by GLE1 in vitro*
**作者**: Prieto, G., et al. (2020)
**摘要**: 通过体外实验验证重组GLE1蛋白与RNA和DDX6等蛋白的相互作用，揭示其在应激颗粒动态组装中的调控功能，强调GLE1-RNA结合结构域的重要性。
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以上文献涵盖GLE1的结构、功能及疾病关联研究，为理解其分子机制提供关键依据。
Recombinant human GLE1 protein is a genetically engineered form of the endogenous GLE1 protein, which plays critical roles in mRNA export from the nucleus and translation regulation. Native GLE1 is essential for the termination phase of mRNA export through the nuclear pore complex and modulates cytoplasmic deadenylation-dependent mRNA turnover. It contains distinct functional domains, including a C-terminal region vital for interacting with the ATPase/RNA helicase DDX19 and a coiled-coil domain mediating oligomerization. GLE1 functions as a dynamic regulator in nucleocytoplasmic transport and stress granule dynamics, with two splice isoforms (GLE1A and GLE1B) displaying differential subcellular localization.
The recombinant version is typically expressed in bacterial or mammalian systems to study its molecular interactions, structural features, and mechanistic contributions to RNA processing. Research on recombinant GLE1 is particularly relevant in neurodegenerative diseases, as GLE1 mutations are linked to lethal congenital contracture syndrome (LCCS1) and amyotrophic lateral sclerosis (ALS). By producing recombinant GLE1, scientists can dissect its role in mRNA metabolism, characterize pathological mutations, and develop therapeutic strategies targeting RNA dysregulation in related disorders. This tool has become indispensable for *in vitro* assays, antibody production, and drug screening studies.
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