| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLIS3 |
| Uniprot No | Q8NEA6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-774aa |
| 氨基酸序列 | MVQRLGLISPPASQVSTACNQISPSLQRAMNAANLNIPPSDTRSLISRESLASTTLSLTESQSASSMKQEWSQGYRALPSLSNHGSQNGLDLGDLLSLPPGTSMSSNSVSNSLPSYLFGTESSHSPYPSPRHSSTRSHSARSKKRALSLSPLSDGIGIDFNTIIRTSPTSLVAYINGSRASPANLSPQPEVYGHFLGVRGSCIPQPRPVPGSQKGVLVAPGGLALPAYGEDGALEHERMQQLEHGGLQPGLVNHMVVQHGLPGPDSQPAGLFKTERLEEFPGSTVDLPPAPPLPPLPPPQGPPPPYHAHAHLHHPELGPHAQQLALPQATLDDDGEMDGIGGKHCYRWIDCSALYDQQEELVRHIEKVHIDQRKGEDFTCFWAGCPRRYKPFNARYKLLIHMRVHSGEKPNKCTFEGCEKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCTKRYTDPSSLRKHVKAHSSKEQQARKKLRSSTEVHPDLLTDCLTVQSLQPATSPRDAAAEGTVGRSPGPGPDLYSAPIFSSNYSSRSGTAAGAVPPPHPVSHPSPGHNVQGSPHNPSSQLPPLTAVDAGAERFAPSAPSPHHISPRRVPAPSSILQRTQPPYTQQPSGSHLKSYQPETNSSFQPNGIHVHGFYGQLQKFCPPHYPDSQRIVPPVSSCSVVPSFEDCLVPTSMGQASFDVFHRAFSTHSGITVYDLPSSSSSLFGESLRSGAEDATFLQISTVDRCPSQLSSVYTEG |
| 分子量 | 85.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GLIS3蛋白的3篇参考文献示例（注：文献为假设性示例，具体内容需根据实际检索验证）：
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1. **文献名称**：*"Recombinant Human GLIS3 Protein Enhances Insulin Gene Transcription in Pancreatic β-Cells"*
**作者**：Kang HS et al.
**摘要**：研究通过在大肠杆菌中表达并纯化重组人GLIS3蛋白，探究其在胰岛β细胞中对胰岛素基因的调控作用。实验表明，GLIS3直接结合胰岛素启动子区域，并通过染色质免疫沉淀验证其功能，提示其在糖尿病治疗中的潜在应用。
2. **文献名称**：*"Structural and Functional Characterization of GLIS3 Zinc Finger Domain via Recombinant Protein Analysis"*
**作者**：Nakashima M et al.
**摘要**：本研究利用昆虫细胞系统表达重组人GLIS3蛋白，解析其锌指结构域的晶体结构，并揭示关键氨基酸残基对DNA结合活性的影响，为GLIS3相关先天性疾病的突变研究提供结构基础。
3. **文献名称**：*"GLIS3 Recombinant Protein Regulates Thyroid Follicular Development in vitro"*
**作者**：Kato T et al.
**摘要**：通过哺乳细胞表达重组GLIS3蛋白，研究其在甲状腺滤泡细胞分化中的作用。实验发现，GLIS3通过激活甲状腺特异性基因（如甲状腺球蛋白）促进滤泡形成，为先天性甲状腺功能异常的机制提供新见解。
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如需获取真实文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词：
- "recombinant human GLIS3 protein"
- "GLIS3 expression and function"
- "GLIS3 structural analysis"
Human GLIS3 (GLI-similar 3) is a zinc finger transcription factor crucial for regulating gene networks involved in embryonic development, cellular differentiation, and metabolic homeostasis. It belongs to the GLI family, sharing structural homology with GLI proteins but functioning independently of Hedgehog signaling. GLIS3 binds to specific DNA sequences (GLIS-binding sites) to activate or repress target genes, playing pivotal roles in pancreatic β-cell development, insulin production, thyroid hormone biosynthesis, and kidney function. Mutations or dysregulation of GLIS3 are linked to congenital hypothyroidism, neonatal diabetes, polycystic kidney disease, and increased susceptibility to type 1/2 diabetes.
Recombinant human GLIS3 protein, typically produced in expression systems like *E. coli* or mammalian cells (e.g., HEK293), enables functional studies of its DNA-binding properties, protein interactions, and regulatory mechanisms. Purified GLIS3 is used in *in vitro* assays to investigate its role in transcription modulation, β-cell regeneration, or epithelial-mesenchymal transition in cancer. Research also focuses on its potential as a therapeutic target for metabolic disorders or as a screening tool for drug candidates aiming to restore GLIS3 activity in diabetes or mitigate its oncogenic effects in malignancies like hepatocellular carcinoma. However, challenges remain in fully deciphering its context-dependent signaling and tissue-specific post-translational modifications.
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