| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR151 |
| Uniprot No | Q8TDV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MLAAAFADSNSSSMNVSFAHLHFAGGYLPSDSQDWRTIIPALLVAVCLVGFVGNLCVIGILLHNAWKGKPSMIHSLILNLSLADLSLLLFSAPIRATAYSKSVWDLGWFVCKSSDWFIHTCMAAKSLTIVVVAKVCFMYASDPAKQVSIHNYTIWSVLVAIWTVASLLPLPEWFFSTIRHHEGVEMCLVDVPAVAEEFMSMFGKLYPLLAFGLPLFFASFYFWRAYDQCKKRGTKTQNLRNQIRSKQVTVMLLSIAIISALLWLPEWVAWLWVWHLKAAGPAPPQGFIALSQVLMFSISSANPLIFLVMSEEFREGLKGVWKWMITKKPPTVSESQETPAGNSEGLPDKVPSPESPASIPEKEKPSSPSSGKGKTEKAEIPILPDVEQFWHERDTVPSVQDNDPIPWEHEDQETGEGVK |
| 分子量 | 73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR151蛋白的3篇参考文献示例(注：以下文献为假设性示例，实际检索请通过学术数据库验证)：
1. **文献名称**：*"Cloning and Functional Characterization of Human GPR151: A Novel GPCR Expressed in Brain Regions Associated with Neuropathic Pain"*
**作者**：Smith A, et al. (2018)
**摘要**：该研究报道了人GPR151基因的克隆及其在HEK293细胞中的重组表达，通过cAMP信号分析揭示其在神经系统中潜在参与疼痛调控的分子机制。
2. **文献名称**：*"Structural Insights into GPR151 Reveal a Unique Ligand-Binding Pocket among Class A GPCRs"*
**作者**：Jin X, et al. (2020)
**摘要**：利用冷冻电镜技术解析了重组人GPR151蛋白的三维结构，发现其配体结合口袋的独特构象，为开发靶向GPR151的神经疾病药物提供结构基础。
3. **文献名称**：*"GPR151 Modulates Dopaminergic Signaling and Addiction-Related Behaviors in Rodent Models"*
**作者**：Brown K, et al. (2019)
**摘要**：通过重组GPR151蛋白体外功能实验和小鼠模型，证明该受体通过调节多巴胺能通路影响药物成瘾行为，提示其作为治疗靶点的潜力。
**建议检索关键词**：
- PubMed/Google Scholar中搜索 **"recombinant human GPR151"、"GPR151 expression and function"、"GPR151 structure"**
- 关注领域内期刊如 *Nature Communications*、*Journal of Biological Chemistry*、*Neuropharmacology*
GPR151 (G protein-coupled receptor 151) is an orphan receptor belonging to the rhodopsin-like subfamily of G protein-coupled receptors (GPCRs). Initially identified through genomic analysis, it remains classified as an orphan due to its unknown endogenous ligand. Predominantly expressed in the central nervous system, particularly in the habenula, thalamus, and hypothalamus, GPR151 is implicated in modulating neuroendocrine functions, addiction-related behaviors, and pain signaling pathways. Its conservation across vertebrate species suggests evolutionary significance in regulating adaptive physiological responses.
Structurally, GPR151 features seven transmembrane domains characteristic of GPCRs, with extracellular N-terminal and intracellular C-terminal regions. Recombinant human GPR151 protein is engineered using expression systems like HEK293 or insect cells, often fused with tags (e.g., FLAG, His-tag) to facilitate purification and detection. The recombinant form enables ligand-binding assays, receptor trafficking studies, and signaling pathway characterization (e.g., coupling to Gαi/o proteins). Recent studies link GPR151 to neurodegenerative and neuropsychiatric disorders, sparking interest in its potential as a therapeutic target. However, ligand identification remains a critical hurdle, limiting drug discovery efforts. Current research leverages recombinant protein tools to elucidate its physiological roles and explore biased signaling mechanisms. Advances in cryo-EM and structural biology using recombinant GPR151 may accelerate deorphanization and mechanistic understanding.
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