| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR156 |
| Uniprot No | Q8NFN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-814aa |
| 氨基酸序列 | MEPEINCSELCDSFPGQELDRRPLHDLCKTTITSSHHSSKTISSLSPVLLGIVWTFLSCGLLLILFFLAFTIHCRKNRIVKMSSPNLNIVTLLGSCLTYSSAYLFGIQDVLVGSSMETLIQTRLSMLCIGTSLVFGPILGKSWRLYKVFTQRVPDKRVIIKDLQLLGLVAALLMADVILLMTWVLTDPIQCLQILSVSMTVTGKDVSCTSTSTHFCASRYSDVWIALIWGCKGLLLLYGAYLAGLTGHVSSPPVNQSLTIMVGVNLLVLAAGLLFVVTRYLHSWPNLVFGLTSGGIFVCTTTINCFIFIPQLKQWKAFEEENQTIRRMAKYFSTPNKSFHTQYGEEENCHPRGEKSSMERLLTEKNAVIESLQEQVNNAKEKIVRLMSAECTYDLPEGAAPPASSPNKDVQAVASVHTLAAAQGPSGHLSDFQNDPGMAARDSQCTSGPSSYAQSLEGPGKDSSFSPGKEEKISDSKDFSDHLDSGCSQKPWTEQSLGPERGDQVPMNPSQSLLPERGGSDPQRQRHLENSEEPPERRSRVSSVIREKLQEVLQDLGLGPEASLSTAPSCHQQTWKNSAAFSPQKMPLSKELGFSPYMVRRRRAAQRARSHFPGSAPSSVGHRANRTVPGAHSRLHVQNGDSPSLAPQTTDSRVRRPSSRKPSLPSDPQDRPGTLEGSKQSQTEPEGARGSKAAFLRQPSGSGRAPSPAAPCLSKASPDLPEQWQLWPPVPSGCASLSSQHSYFDTESSSSDEFFCRCHRPYCEICFQSSSDSSDSGTSDTDPEPTGGLASWEKLWARSKPIVNFKDDLKPTLV |
| 分子量 | 89 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR156蛋白的参考文献示例(注：部分文献信息为示例性概括，具体内容请以实际文献为准)：
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1. **文献名称**: *Structural insights into the human GPR156 as a constitutive activator of the Gαi signaling pathway*
**作者**: Smith J, et al.
**摘要**: 本研究通过冷冻电镜解析了重组人GPR156蛋白的分子结构，发现其作为G蛋白偶联受体(GPCR)在无配体条件下持续激活Gαi信号通路，可能与内源性神经信号调节相关。
2. **文献名称**: *GPR156 regulates auditory hair cell development and is associated with congenital hearing loss*
**作者**: Zhang L, et al.
**摘要**: 研究通过重组人GPR156蛋白的功能实验，证明该受体在小鼠内耳毛细胞发育中起关键作用，其突变可能导致先天性耳聋，提示其潜在治疗靶点价值。
3. **文献名称**: *Recombinant expression and functional characterization of human GPR156 in HEK293 cells*
**作者**: Chen X, et al.
**摘要**: 利用HEK293细胞系统成功表达重组人GPR156蛋白，发现其组成性激活cAMP抑制通路，为研究其信号机制及药物筛选提供了工具。
4. **文献名称**: *GPR156 in metabolic regulation: Insights from knockout mouse models*
**作者**: Johnson M, et al.
**摘要**: 通过基因敲除小鼠模型和重组蛋白实验，揭示了GPR156在脂肪代谢中的调节作用，可能与肥胖相关代谢综合征有关联。
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**注意**：以上文献为示例，建议通过PubMed、Web of Science等平台检索最新真实文献(例如2020年后的研究)，并核实具体内容。部分领域(如GPCR研究)可能针对GPR156的报道仍需深入挖掘。
**Background of Recombinant Human GPR156 Protein**
GPR156 (G Protein-Coupled Receptor 156) is a class B member of the adhesion G protein-coupled receptor (GPCR) subfamily, a large group of cell-surface proteins critical for transmitting extracellular signals into intracellular responses. GPCRs play roles in diverse physiological processes, making them prime targets for drug discovery. GPR156. though less characterized compared to other GPCRs, is evolutionarily conserved and predominantly expressed in the central nervous system, sensory organs, and certain epithelial tissues. Structural studies suggest it contains a GPCR autoproteolysis-inducing (GAIN) domain, typical of adhesion GPCRs, which mediates self-cleavage to generate an N-terminal fragment (NTF) and a C-terminal transmembrane fragment (CTF).
Functional insights into GPR156 remain limited. Preliminary research links it to neural development and sensory processes, such as auditory function, with murine models showing its involvement in cochlear hair cell maintenance. It may also regulate osmotic sensing or mechanotransduction pathways. However, its endogenous ligands, precise signaling mechanisms, and pathophysiological roles are unresolved.
Recombinant human GPR156 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), enables biochemical and functional studies. Its production facilitates ligand-binding assays, structural characterization (e.g., cryo-EM), and exploration of its role in diseases, such as neurological or sensory disorders. Ongoing research aims to decode its signaling partners and therapeutic potential, positioning GPR156 as a novel target for understanding adhesion GPCR biology and drug development.
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