| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR30 |
| Uniprot No | Q99527 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDVTSQARGVGLEMYPGTAQPAAPNTTSPELNLSHPLLGTALANGTGELSEHQQYVIGLFLSCLYTIFLFPIGFVGNILILVVNISFREKMTIPDLYFINLAVADLILVADSLIEVFNLHERYYDIAVLCTFMSLFLQVNMYSSVFFLTWMSFDRYIALARAMRCSLFRTKHHARLSCGLIWMASVSATLVPFTAVHLQHTDEACFCFADVREVQWLEVTLGFIVPFAIIGLCYSLIVRVLVRAHRHRGLRPRRQKALRMILAVVLVFFVCWLPENVFISVHLLQRTQPGAAPCKQSFRHAHPLTGHIVNLAAFSNSCLNPLIYSFLGETFRDKLRLYIEQKTNLPALNRFCHAALKAVIPDSTEQSDVRFSSAV |
| 分子量 | 68.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR30蛋白的3-4篇参考文献及其简要摘要内容：
1. **文献名称**：*"A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling"*
**作者**：Revankar, C. M. et al.
**摘要**：该研究首次证实GPR30(现称GPER)作为跨膜雌激素受体，能在细胞膜上介导快速的雌激素信号传递。实验通过重组人GPR30蛋白的表达，证明其与雌激素特异性结合并激活下游信号通路(如cAMP和钙离子信号)。
2. **文献名称**：*"Identity of an Estrogen Membrane Receptor Coupled to a G Protein in Human Breast Cancer Cells"*
**作者**：Thomas, P. et al.
**摘要**：研究利用重组人GPR30蛋白模型，阐明其在乳腺癌细胞中通过G蛋白偶联机制调节雌激素的非基因组效应，包括细胞迁移和增殖，为GPR30在肿瘤生物学中的作用提供依据。
3. **文献名称**：*"The G Protein-Coupled Receptor GPR30 Mediates the Proliferative Effects of Estrogen in Cancer Cells"*
**作者**：Filardo, E. J. et al.
**摘要**：通过重组GPR30蛋白的功能分析，揭示了其介导雌激素诱导的癌细胞增殖和MAPK/ERK信号激活的关键作用，强调了GPR30作为治疗靶点的潜力。
4. **文献名称**：*"GPR30: A G Protein-Coupled Receptor for Estrogen in the Cardiovascular System"*
**作者**：Prossnitz, E. R. & Arterburn, J. B.
**摘要**：综述了重组GPR30蛋白在心血管系统中的研究进展，包括其参与雌激素介导的血管保护机制，并讨论了重组蛋白在药物筛选和机制研究中的应用。
以上文献覆盖了重组人GPR30蛋白在信号机制、疾病模型及治疗潜力中的核心研究。
GPR30. also known as G protein-coupled estrogen receptor (GPER), is a transmembrane receptor identified in the early 2000s as a novel mediator of estrogen signaling. Unlike classical estrogen receptors (ERα and ERβ), which function as nuclear transcription factors, GPR30 primarily operates through non-genomic pathways, initiating rapid cellular responses such as cAMP production, calcium mobilization, and activation of MAPK/ERK signaling cascades. It is broadly expressed in tissues, including the cardiovascular, nervous, and reproductive systems, and has been implicated in hormone-dependent physiological processes and diseases like cancer, cardiovascular disorders, and metabolic syndromes.
Recombinant human GPR30 protein is engineered using heterologous expression systems (e.g., mammalian cells or bacteria) to produce a purified, bioactive form for experimental studies. This protein retains the structural hallmarks of native GPR30. including seven transmembrane domains critical for ligand binding and G-protein coupling. Researchers utilize recombinant GPR30 to dissect its signaling mechanisms, screen targeted therapeutics, and explore its interplay with classical estrogen pathways. Notably, its role in mediating estrogenic effects in ER-negative contexts has spurred interest in cancer drug resistance and sex-specific disease disparities. Current challenges include clarifying its ligand specificity and resolving conflicting data about its physiological relevance, underscoring the need for standardized recombinant tools to advance translational research.
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