| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIPAP1 |
| Uniprot No | Q4V328 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-841aa |
| 氨基酸序列 | MAQALSEEEFQRMQAQLLELRTNNYQLSDELRKNGVELTSLRQKVAYLDKEFSKAQKALSKSKKAQEVEVLLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQMEQLEQENQQLKEGAAGAGVAQAGPLVDGELLRLQAENTALQKNVAALQERYGKEAGKFSAVSEGQGDPPGGPAPTVLAPMPLAEVELKWEMEKEEKRLLWEQLQGLESSKQAETSRLQEELAKLSEKLKKKQESFCRLQTEKETLFNDSRNKIEELQQRKEADHKAQLARTQKLQQELEAANQSLAELRDQRQGERLEHAAALRALQDQVSIQSADAQEQVEGLLAENNALRTSLAALEQIQTAKTQELNMLREQTTGLAAELQQQQAEYEDLMGQKDDLNSQLQESLRANSRLLEQLQEIGQEKEQLTQELQEARKSAEKRKAMLDELAMETLQEKSQHKEELGAVRLRHEKEVLGVRARYERELRELHEDKKRQEEELRGQIREEKARTRELETLQQTVEELQAQVHSMDGAKGWFERRLKEAEESLQQQQQEQEEALKQCREQHAAELKGKEEELQDVRDQLEQAQEERDCHLKTISSLKQEVKDTVDGQRILEKKGSAALKDLKRQLHLERKRADKLQERLQDILTNSKSRSGLEELVLSEMNSPSRTQTGDSSSISSFSYREILREKESSAVPARSLSSSPQAQPPRPAELSDEEVAELFQRLAETQQEKWMLEEKVKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDRSGLGSVLRDLVKPGDENLREMNKKLQNMLEEQLTKNMHLHKDMEVLSQEIVRLSKECVGPPDPDLEPGETS |
| 分子量 | 122.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是围绕重组人GRIPAP1蛋白的模拟参考文献(非真实文献，仅供参考格式)：
1. **文献名称**：*Structural and functional analysis of human GRIPAP1 in neuronal synaptic plasticity*
**作者**：Dong, X. et al.
**摘要**：研究通过重组表达人GRIPAP1蛋白，揭示其通过C端结构域与AMPA受体相互作用，调节突触后膜受体的稳定性，影响神经元信号传递。
2. **文献名称**：*Recombinant GRIPAP1 promotes cell migration via PI3K/AKT pathway in breast cancer cells*
**作者**：Chen, L. et al.
**摘要**：利用哺乳动物细胞表达系统纯化GRIPAP1蛋白，发现其通过激活PI3K/AKT通路增强乳腺癌细胞迁移，提示其在癌症转移中的潜在作用。
3. **文献名称**：*GRIPAP1 interaction with kinesin motors modulates intracellular cargo transport*
**作者**：Yamamoto, S. et al.
**摘要**：通过重组蛋白结合免疫共沉淀实验，证实GRIPAP1与驱动蛋白KIF5B结合，影响线粒体等细胞器的定向运输。
4. **文献名称**：*Expression and purification of recombinant GRIPAP1 in E. coli: Implications for neurodegenerative diseases*
**作者**：Wang, Q. et al.
**摘要**：优化了原核表达系统高效制备可溶性GRIPAP1蛋白的方法，并发现其异常聚集可能与神经退行性疾病相关。
注：以上为模拟内容，实际文献需通过学术数据库(如PubMed、Google Scholar)以“GRIPAP1”或“Gripap1”为关键词检索。
Gripap1 (GRIP1-associated protein 1), also known as GRASP-1. is a multidomain scaffolding protein predominantly expressed in the brain. It interacts with glutamate receptor-interacting protein 1 (GRIP1), which anchors AMPA receptors at postsynaptic membranes, implicating its role in synaptic plasticity and neuronal signaling. Structurally, GRIPAP1 contains PDZ domains, a major binding motif for protein-protein interactions, enabling its coordination with synaptic receptors, kinases, and trafficking machinery.
Studies suggest GRIPAP1 regulates AMPA receptor trafficking and dendritic spine morphology, influencing learning and memory processes. Dysregulation has been linked to neurodevelopmental disorders and neurodegenerative conditions. Recombinant human GRIPAP1 protein, typically produced in E. coli or mammalian systems via gene cloning and affinity purification, serves as a critical tool for studying synaptic protein networks. It facilitates in vitro binding assays, antibody development, and mechanistic explorations of neuronal communication pathways. Recent research also highlights potential cross-talk with cancer-related pathways, though its oncogenic roles remain under investigation. As a versatile interactor in neural and non-neural tissues, GRIPAP1 continues to garner interest in both basic neuroscience and therapeutic discovery.
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