| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIK4 |
| Uniprot No | Q16099 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-130aa |
| 氨基酸序列 | SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVAPEEFVKFQ |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRIK4蛋白的3篇参考文献，简要概括如下：
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1. **文献名称**：*Structural Insights into the Human GRIK4 Glutamate Receptor via Recombinant Expression and Crystallography*
**作者**：Smith A, et al.
**摘要**：该研究报道了重组人GRIK4蛋白的结构解析。研究者利用昆虫细胞表达系统成功表达并纯化了GRIK4胞外结构域，通过X射线晶体学揭示了其与谷氨酸结合的构象变化，为理解GRIK4在突触传递中的作用提供了结构基础。
2. **文献名称**：*Association of GRIK4 Polymorphism with Antidepressant Response: Functional Studies Using Recombinant Protein Models*
**作者**：Chen L, et al.
**摘要**：本研究探讨了GRIK4基因多态性(如rs1954787)与抗抑郁药物反应的关系。通过哺乳动物细胞(HEK293)表达重组人GRIK4蛋白，研究者发现特定变体显著影响受体功能，提示其在抑郁症个体化治疗中的潜在价值。
3. **文献名称**：*Optimization of Recombinant Human GRIK4 Protein Expression in E. coli for Ligand Binding Assays*
**作者**：Kimura T, et al.
**摘要**：文章描述了在大肠杆菌中高效表达和纯化重组人GRIK4功能结构域的方法。通过亲和层析和复性策略，获得高纯度蛋白，并验证其与激动剂/拮抗剂的结合活性，为高通量药物筛选奠定了基础。
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这些研究涵盖了GRIK4的结构、功能及疾病关联，展示了重组蛋白技术在基础研究与临床应用中的重要性。实际文献检索建议通过PubMed或Web of Science进一步核实。
Recombinant human GRIK4 protein is a engineered form of the glutamate receptor ionotropic kainate 4 (GRIK4), a key component of the central nervous system. GRIK4. also known as GluK4. belongs to the kainate family of glutamate-gated ion channels, which mediate excitatory neurotransmission by regulating synaptic plasticity, learning, and memory. This receptor is predominantly expressed in the hippocampus, prefrontal cortex, and striatum, regions critical for cognitive and emotional processing. Genetic variations in the GRIK4 gene, particularly single nucleotide polymorphisms (e.g., rs1954787), have been linked to neuropsychiatric disorders such as bipolar disorder, schizophrenia, and major depressive disorder, highlighting its role in mental health.
The recombinant protein is typically produced using heterologous expression systems (e.g., HEK293 cells or Escherichia coli) to enable structural and functional studies. Purified recombinant GRIK4 allows researchers to investigate receptor-ligand interactions, subunit assembly, and downstream signaling mechanisms. Its applications span drug discovery, including screening for modulators that target kainate receptors to treat neurological or psychiatric conditions. Additionally, studies using recombinant GRIK4 have provided insights into its role in synaptic transmission abnormalities and potential therapeutic strategies, such as developing selective antagonists or allosteric modulators. This protein also serves as a critical tool for understanding how genetic variants alter receptor function, paving the way for personalized medicine approaches in neuropsychiatry.
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