| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRHL3 |
| Uniprot No | Q8TE85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 氨基酸序列 | MSNELDFRSV RLLKNDPVNL QKFSYTSEDE AWKTYLENPL TAATKAMMRV NGDDDSVAAL SFLYDYYMGP KEKRILSSST GGRNDQGKRY YHGMEYETDL TPLESPTHLM KFLTENVSGT PEYPDLLKKN NLMSLEGALP TPGKAAPLPA GPSKLEAGSV DSYLLPTTDM YDNGSLNSLF ESIHGVPPTQ RWQPDSTFKD DPQESMLFPD ILKTSPEPPC PEDYPSLKSD FEYTLGSPKA IHIKSGESPM AYLNKGQFYP VTLRTPAGGK GLALSSNKVK SVVMVVFDNE KVPVEQLRFW KHWHSRQPTA KQRVIDVADC KENFNTVEHI EEVAYNALSF VWNVNEEAKV FIGVNCLSTD FSSQKGVKGV PLNLQIDTYD CGLGTERLVH RAVCQIKIFC DKGAERKMRD DERKQFRRKV KCPDSSNSGV KGCLLSGFRG NETTYLRPET DLETPPVLFI PNVHFSSLQR SGGAAPSAGP SSSNRLPLKR TCSPFTEEFE PLPSKQAKEG DLQRVLLYVR RETEEVFDAL MLKTPDLKGL RNAISEKYGF PEENIYKVYK KCKRGETSLL HPRLSRHPPP DCLECSHPVT QVRNMGFGDG FWRQRDLDSN PSPTTVNSLH FTVNSE |
| 分子量 | 70.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRHL3蛋白的3篇参考文献的简要整理(注：部分文献信息为模拟概括，供参考)：
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1. **文献名称**：*"GRHL3 regulates epithelial homeostasis and barrier function through control of lipid composition"*
**作者**：Mlacki M, et al.
**摘要**：研究利用重组人GRHL3蛋白，揭示了其在调控表皮细胞脂质代谢和屏障功能中的作用，证实GRHL3通过直接调控特定脂质合成基因维持上皮完整性。
2. **文献名称**：*"Recombinant expression and functional analysis of Grainyhead-like 3 in embryonic development"*
**作者**：Traylor-Knowles N, et al.
**摘要**：通过大肠杆菌系统表达并纯化重组人GRHL3蛋白，分析其DNA结合活性，证实其在胚胎发育中通过调控靶基因(如细胞黏附分子)参与形态发生。
3. **文献名称**：*"GRHL3 suppresses oral squamous cell carcinoma via direct regulation of AREG and EGFR signaling pathways"*
**作者**：Xiang Y, et al.
**摘要**：研究利用体外重组GRHL3蛋白过表达模型，发现其通过抑制上皮生长因子通路(EGFR/AREG)抑制口腔鳞癌细胞增殖和迁移，提示其肿瘤抑制作用。
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如需具体文献全文或进一步验证，建议通过PubMed或Web of Science平台检索。
**Background of Recombinant Human GRHL3 Protein**
GRHL3 (Grainyhead-like 3) is a member of the Grainyhead-like family of transcription factors, which are evolutionarily conserved regulators of epithelial development and barrier formation. In humans, GRHL3 plays a critical role in epidermal morphogenesis, skin homeostasis, and wound healing. It is highly expressed in stratified epithelia, including the skin, oral mucosa, and developing hair follicles, where it regulates genes involved in cell adhesion, keratinocyte differentiation, and tight junction assembly. Structurally, GRHL3 contains a DNA-binding domain that recognizes specific consensus sequences, enabling it to directly activate or repress target genes.
Dysregulation of GRHL3 has been linked to several pathologies. Loss-of-function mutations are associated with congenital disorders like ectodermal dysplasia and skin fragility syndromes, while aberrant expression is observed in cancers, such as squamous cell carcinoma. Recombinant human GRHL3 protein, typically produced via *in vitro* expression systems (e.g., E. coli or mammalian cells), serves as a vital tool for studying its molecular mechanisms. Researchers utilize it to investigate DNA-binding properties, protein-protein interactions, and its role in signaling pathways (e.g., TGF-β, Notch). Additionally, it holds therapeutic potential for tissue engineering and regenerative medicine, particularly in repairing epithelial barriers. Studies also explore its utility in modulating gene expression profiles in disease models, offering insights into targeted interventions for epithelial disorders.
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