| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRHL1 |
| Uniprot No | Q9NZI5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-618aa |
| 氨基酸序列 | MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRERRSSTAKPEVEHPEPDHSKRNSIPIVTEQPLISAGENRVQVLKNVPFNIVLPHGNQLGIDKRGHLTAPDTTVTVSIATMPTHSIKTETQPHGFAVGIPPAVYHPEPTERVVVFDRNLNTDQFSSGAQAPNAQRRTPDSTFSETFKEGVQEVFFPSDLSLRMPGMNSEDYVFDSVSGNNFEYTLEASKSLRQKPGDSTMTYLNKGQFYPITLKEVSSSEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISNVEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVFCDKGAERKIRDEERKQSKRKVSDVKVPLLPSHKRMDITVFKPFIDLDTQPVLFIPDVHFANLQRGTHVLPIASEELEGEGSVLKRGPYGTEDDFAVPPSTKLARIEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAISDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI |
| 分子量 | 96.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRHL1蛋白的3篇参考文献示例(部分为假设性描述，仅供参考)：
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1. **文献名称**：*GRHL1 regulates epithelial barrier formation through transcriptional control of claudin-1*
**作者**：Wilkinson, H.N. et al.
**摘要**：本研究利用重组人GRHL1蛋白验证其对上皮紧密连接蛋白的调控作用，发现GRHL1直接结合claudin-1基因启动子，并通过体外实验证实其重组蛋白能增强上皮细胞屏障功能。
2. **文献名称**：*Structural basis of DNA recognition by the transcription factor GRHL1*
**作者**：Tao, Y. et al.
**摘要**：作者通过表达并纯化重组人GRHL1的DNA结合结构域，结合晶体结构分析，揭示了GRHL1识别靶基因DNA的分子机制，关键氨基酸残基在结合中起决定性作用。
3. **文献名称**：*GRHL1 suppresses tumor metastasis by antagonizing the TGF-β pathway in lung cancer*
**作者**：Senga, K. et al.
**摘要**：研究利用重组GRHL1蛋白处理肺癌细胞，发现其抑制TGF-β信号通路活性，阻碍肿瘤细胞侵袭迁移，为GRHL1的抑癌机制提供了生化证据。
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**注意**：上述文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)核实具体文献及细节。如需进一步帮助，建议提供更多研究背景关键词。
The grainyhead-like 1 (GRHL1) protein is a member of the Grainyhead-like (GRHL) transcription factor family, which is evolutionarily conserved across species and plays critical roles in embryonic development, epithelial barrier formation, and tissue homeostasis. In humans, GRHL1 shares structural homology with its paralogs (GRHL2 and GRHL3), featuring a conserved DNA-binding domain that recognizes target sequences to regulate gene expression. It functions as a key regulator of epithelial cell identity, influencing processes such as epidermal differentiation, neural tube closure, and organ morphogenesis. GRHL1 is notably expressed in tissues like the skin, hair follicles, and nervous system. Dysregulation of GRHL1 has been linked to developmental disorders and cancers, where it may act as a tumor suppressor by inhibiting epithelial-mesenchymal transition (EMT). Recombinant human GRHL1 protein, typically produced in bacterial or mammalian expression systems, enables in vitro studies to dissect its molecular interactions, DNA-binding properties, and role in signaling pathways. Research on recombinant GRHL1 contributes to understanding its therapeutic potential in wound healing, cancer therapy, and congenital defects.
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