| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRAP |
| Uniprot No | Q13588 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-217aa |
| 氨基酸序列 | MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGVEGFIPK NYIRVKPHPW YSGRISRQLA EEILMKRNHL GAFLIRESES SPGEFSVSVN YGDQVQHFKV LREASGKYFL WEEKFNSLNE LVDFYRTTTI AKKRQIFLRD EEPLLKSPGA CFAQAQFDFS AQDPSQLSFR RGDIIEVLER PDPHWWRGRS CGRVGFFPRS YVQPVHL |
| 分子量 | 25.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRAP蛋白的模拟参考文献示例(注：文献为虚构，仅作格式参考)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant Human GRAP Protein in EGFR Signaling"*
**作者**: Zhang, L.; Wang, H.; Chen, J.
**摘要**: 本研究成功在大肠杆菌中表达了重组人GRAP蛋白，并通过Pull-down实验验证了其与EGFR信号通路中关键蛋白的相互作用。结果表明，重组GRAP能有效抑制下游ERK磷酸化，提示其在调控细胞增殖中的潜在作用。
2. **标题**: *"Structural Insights into GRAP Adaptor Protein and Its Role in T-Cell Receptor Signaling"*
**作者**: Kumar, S.; Lee, M.; Kim, D.
**摘要**: 通过X射线晶体学解析了重组人GRAP蛋白的三维结构，揭示了其SH2和SH3结构域的关键结合位点。功能实验表明，重组GRAP通过调节T细胞受体(TCR)信号中的相互作用，影响免疫细胞活化。
3. **标题**: *"Recombinant Human GRAP as a Therapeutic Inhibitor in Breast Cancer Metastasis"*
**作者**: Smith, R.; Patel, A.; Garcia, E.
**摘要**: 研究利用重组人GRAP蛋白探究其在乳腺癌细胞迁移中的作用。体外实验表明，GRAP过表达显著抑制MAPK信号传导，降低癌细胞侵袭能力，提示其作为治疗靶点的潜力。
4. **标题**: *"Development of a GRAP Knockdown Model Using Recombinant Protein-Based CRISPR/Cas9 Delivery"*
**作者**: Oliveira, T.; Müller, P.
**摘要**: 构建了基于重组人GRAP蛋白的CRISPR递送系统，用于靶向敲低肿瘤细胞中的GRAP基因。实验证明该方法可有效沉默基因表达，并验证了GRAP在调控细胞凋亡中的功能。
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如需真实文献，建议在学术数据库(如PubMed、Web of Science)检索关键词“recombinant human GRAP protein”或“GRAP adaptor protein signaling”。
**Background of Recombinant Human GRAP Protein**
The GRAP (GRB2-related adaptor protein) is a cytosolic signaling adapter protein involved in regulating intracellular signal transduction pathways, particularly those downstream of receptor tyrosine kinases and immune receptors. Structurally, GRAP contains a C-terminal SH3 domain and multiple proline-rich motifs, enabling interactions with partner proteins like GRB2 and SOS1 to modulate Ras-MAPK signaling cascades. It plays critical roles in T-cell receptor (TCR) signaling, thymocyte development, and immune response regulation.
Recombinant human GRAP protein is engineered using expression systems such as *E. coli* or mammalian cells, ensuring post-translational modifications similar to native protein when required. Its production enables biochemical and functional studies to dissect signaling mechanisms in normal physiology and disease. Dysregulation of GRAP has been implicated in autoimmune disorders, cancers, and immune dysfunctions, highlighting its therapeutic and diagnostic potential. Researchers utilize recombinant GRAP to investigate protein-protein interactions, screen inhibitors, or develop assays for pathway activation profiling. Its applications extend to understanding pathologies linked to aberrant immune signaling and evaluating targeted therapies aiming to restore cellular communication networks. Ongoing research focuses on GRAP's role in fine-tuning immune activation thresholds, offering insights into precision immunomodulation strategies.
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