| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPSM2 |
| Uniprot No | P81274 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-684aa |
| 氨基酸序列 | MEENLISMRE DHSFHVRYRM EASCLELALE GERLCKSGDC RAGVSFFEAA VQVGTEDLKT LSAIYSQLGN AYFYLHDYAK ALEYHHHDLT LARTIGDQLG EAKASGNLGN TLKVLGNFDE AIVCCQRHLD ISRELNDKVG EARALYNLGN VYHAKGKSFG CPGPQDVGEF PEEVRDALQA AVDFYEENLS LVTALGDRAA QGRAFGNLGN THYLLGNFRD AVIAHEQRLL IAKEFGDKAA ERRAYSNLGN AYIFLGEFET ASEYYKKTLL LARQLKDRAV EAQSCYSLGN TYTLLQDYEK AIDYHLKHLA IAQELNDRIG EGRACWSLGN AYTALGNHDQ AMHFAEKHLE ISREVGDKSG ELTARLNLSD LQMVLGLSYS TNNSIMSENT EIDSSLNGVR PKLGRRHSME NMELMKLTPE KVQNWNSEIL AKQKPLIAKP SAKLLFVNRL KGKKYKTNSS TKVLQDASNS IDHRIPNSQR KISADTIGDE GFFDLLSRFQ SNRMDDQRCC LQEKNCHTAS TTTSSTPPKM MLKTSSVPVV SPNTDEFLDL LASSQSRRLD DQRASFSNLP GLRLTQNSQS VLSHLMTNDN KEADEDFFDI LVKCQGSRLD DQRCAPPPAT TKGPTVPDED FFSLILRSQG KRMDEQRVLL QRDQNRDTDF GLKDFLQNNA LLEFKNSGKK SADH |
| 分子量 | 76.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例，基于常见研究方向的合理推测，但可能不对应实际存在的文献：
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1. **“Functional characterization of recombinant human GPSM2 in G-protein signaling regulation”**
*Author: Siderovski, D.P. et al.*
摘要：本研究通过在大肠杆菌中表达并纯化重组人GPSM2蛋白，验证其通过GoLoco结构域与Gαi亚基特异性结合，并调控G蛋白信号通路的活性，揭示了GPSM2在细胞信号转导中的分子机制。
2. **“GPSM2 governs cochlear hair cell polarity via interactions with G-proteins”**
*Author: Etheridge, S.L. et al.*
摘要：利用哺乳动物细胞系表达重组人GPSM2蛋白，证明其通过调控细胞极性复合物的定位，影响内耳毛细胞微管结构的形成，为GPSM2突变导致听力障碍提供了分子基础。
3. **“Structural insights into the GoLoco domain of GPSM2 by X-ray crystallography”**
*Author: Yadav, M.K. et al.*
摘要：通过重组表达人GPSM2的GoLoco结构域并解析其晶体结构，揭示了该蛋白与Gαi的结合界面及构象变化，为设计靶向GPSM2的小分子药物提供结构依据。
4. **“GPSM2 depletion disrupts asymmetric cell division in cancer stem cells”**
*Author: Smith, M.C. et al.*
摘要：采用重组GPSM2蛋白进行体外挽救实验，证明其通过稳定NuMA复合体维持肿瘤干细胞的不对称分裂，提示GPSM2在癌症治疗中的潜在靶点作用。
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**注**：以上文献为模拟示例，实际引用时需核实真实来源及准确性。建议通过PubMed、Web of Science等数据库以关键词“GPSM2 recombinant”“GPSM2 signaling”等检索最新文献。
**Background of Recombinant Human GPSM2 Protein**
G-protein signaling modulator 2 (GPSM2), also known as LGN or AGS3-like protein, is a critical regulator of G-protein-coupled receptor (GPCR) signaling pathways. It belongs to the GPSM family, characterized by conserved GoLoco motifs that interact with Gαi/o subunits to modulate G-protein cycling, influencing cell polarity, asymmetric division, and mitotic spindle orientation during development. GPSM2 is particularly vital in neurosensory systems, including inner ear hair cell development, and mutations in *GPSM2* are linked to human disorders like Chudley-McCullough syndrome (CMS), characterized by hearing loss and neurodevelopmental defects.
Recombinant human GPSM2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for biochemical and cell-based studies. It enables researchers to explore GPSM2’s role in GPCR signaling, mitotic regulation, and interactions with binding partners (e.g., Gαi, NuMA). Studies using recombinant GPSM2 have advanced understanding of its structure-function relationships, particularly how GoLoco motifs mediate Gαi binding to control downstream processes. This protein also serves as a tool for investigating pathogenic mechanisms in CMS and potential therapeutic strategies targeting GPCR-related pathways. Its applications span structural biology, drug discovery, and disease modeling, highlighting its significance in both basic and translational research.
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