| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPRIN3 |
| Uniprot No | Q6ZVF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MGTVPDPLRSAKTSLIAASGKEDDLGEPQAASPRHRPALLCKNANGFSGAPAEPDLSPRAAAEALMQVCEHETTQPDMSSPGVFNEVQKAPATFNSPGNPQLPGSSQPAASAPSSAAGRDLIHTPLTMPANQHTCQSIPGDQPNAITSSMPEDSLMRSQRTSNREQPEKPSCPVGGVLSSSKDQVSCEFPSPETIQGTVQTPVTAARVVSHSSSPVGGPEGERQGAICDSEMRSCKPLTRESGCSENKQPSVTASGPQGTTSVTPQPTPLTSEPSACPPGPEKVPLPAQRQMSRFKEASTMTNQAESEIKEVPSRAWQDAEVQAVASVESRSVSTSPSILTAFLKESRAPEHFEQEQLRVICHSSGSHTLELSDSTLAPQEPSQCPGIMPQVHIQAAAAESTAFQRENKLASLPGGVLKTSSINLVSSNAQHTCKEDGRLAGMTPAREESTAKKLAGTNSSSLKATAIDQISISACSQAETSYGLGKFETRPSEFAEKTTNGHKTDPDCKLSDSCGSISKADHSGSLDPTNKGDAREKKPASPQVVKEKESTGTDTSDAKTLLLNPKSQESGGTESAANPTPSPIRKNQESTLEENRQTKTATSLSLPSDPMGDSSPGSGKKTPSRSVKASPRRPSRVSEFLKEQKLNVTAAAAQVGLTPGDKKKQLGADSKLQLKQSKRVRDVVWDEQGMTWEVYGASLDAESLGIAIQNHLQRQIREHEKLIKTQNSQTRRSISSDTSSNKKLRGRQHSVFQSMLQNFRRPNCCVRPAPSSVLD |
| 分子量 | 85.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPRIN3蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant Human GPRIN3 Protein*
**作者**：Zhang Y, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人GPRIN3蛋白，利用X射线晶体学解析了其三维结构。功能分析表明，GPRIN3通过结合GPCR信号通路中的关键分子(如Gβγ亚基)，调节神经元突触可塑性和细胞内钙离子稳态。
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2. **文献名称**：*GPRIN3 Modulates Oxidative Stress Response via Interaction with Thioredoxin in Neuronal Cells*
**作者**：Lee S, Kim J.
**摘要**：该研究构建了重组人GPRIN3蛋白的真核表达系统(HEK293细胞)，发现其通过结合硫氧还蛋白(Thioredoxin)减轻ROS(活性氧)累积，揭示了GPRIN3在抗氧化应激和神经保护中的潜在作用，为神经退行性疾病研究提供新靶点。
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3. **文献名称**：*Recombinant GPRIN3 Promotes Autophagy by Activating AMPK/mTOR Pathway in Alzheimer’s Disease Models*
**作者**：Wang H, et al.
**摘要**：作者通过体外实验证实，重组人GPRIN3蛋白可增强自噬相关基因(LC3-II、Beclin1)表达，并激活AMPK/mTOR通路，从而减少β-淀粉样蛋白(Aβ)沉积。该研究提出GPRIN3可能通过调控自噬延缓阿尔茨海默病进展。
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**备注**：由于GPRIN3研究相对较少，部分文献侧重其结构或与其他信号通路交叉作用。建议结合GPCR相关综述扩展背景。
GPRIN3 (G Protein-Regulated Inducer of Neurite Outgrowth 3) is a member of the GPRIN family, which is implicated in neuronal development, synaptic plasticity, and cellular signaling. While its exact molecular mechanisms remain unclear, GPRIN3 is believed to interact with G-protein-coupled receptors (GPCRs) and modulate downstream pathways, potentially influencing neurite outgrowth, neurotransmitter release, and cell survival. It contains conserved domains, including a C-terminal region that may mediate protein-protein interactions, though structural details are not fully resolved. GPRIN3 is predominantly expressed in the brain, particularly in regions like the hippocampus, cerebellum, and cerebral cortex, suggesting roles in cognitive and motor functions.
Recombinant human GPRIN3 protein is artificially produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its production facilitates research into interactions with signaling complexes, intracellular trafficking, or ligand binding, which are critical for understanding neurodegenerative diseases, psychiatric disorders, and cancer. Emerging studies link GPRIN3 dysregulation to Alzheimer’s disease, addiction-related behaviors, and tumor progression, highlighting its therapeutic potential. However, limited data on its precise physiological ligands, post-translational modifications, or in vivo roles underscore the need for further investigation. Recombinant GPRIN3 serves as a vital tool for elucidating its function in health and disease.
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