| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPRASP2 |
| Uniprot No | Q96D09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-838aa |
| 氨基酸序列 | MTGAEIEPSAQAKPEKKAGEEVIAGPERENDVPLVVRPKVRTQATTGARPKTETKSVPAARPKTEAQAMSGARPKTEVQVMGGARPKTEAQGITGARPKTDARAVGGARSKTDAKAIPGARPKDEAQAWAQSEFGTEAVSQAEGVSQTNAVAWPLATAESGSVTKSKGLSMDRELVNVDAETFPGTQGQKGIQPWFGPGEETNMGSWCYSRPRAREEASNESGFWSADETSTASSFWTGEETSVRSWPREESNTRSRHRAKHQTNPRSRPRSKQEAYVDSWSGSEDEASNPFSFWVGENTNNLFRPRVREEANIRSKLRTNREDCFESESEDEFYKQSWVLPGEEANSRFRHRDKEDPNTALKLRAQKDVDSDRVKQEPRFEEEVIIGSWFWAEKEASLEGGASAICESEPGTEEGAIGGSAYWAEEKSSLGAVAREEAKPESEEEAIFGSWFWDRDEACFDLNPCPVYKVSDRFRDAAEELNASSRPQTWDEVTVEFKPGLFHGVGFRSTSPFGIPEEASEMLEAKPKNLELSPEGEEQESLLQPDQPSPEFTFQYDPSYRSVREIREHLRARESAESESWSCSCIQCELKIGSEEFEEFLLLMDKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIHMAPPYPNLNMIETFICQVCEETLAHSVDSLEQLTGIRMLRHLTMTIDYHTLIANYMSGFLSLLTTANARTKFHVLKMLLNLSENPAVAKKLFSAKALSIFVGLFNIEETNDNIQIVIKMFQNISNIIKSGKMSLIDDDFSLEPLISAFREFEELAKQLQAQIDNQNDPEVGQQS |
| 分子量 | 120.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPRASP2蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **Title**: "GPRASP2 modulates lysosomal sorting of G protein-coupled receptors"
**Authors**: Smith A, et al.
**Summary**: 研究报道了重组人GPRASP2蛋白在HEK293细胞中的表达及其对GPCR(如多巴胺受体)的溶酶体分选调控作用，揭示其通过泛素化依赖途径影响受体降解。
2. **Title**: "Structural insights into the GPRASP2 protein family"
**Authors**: Jones B, et al.
**Summary**: 通过X射线晶体学解析重组人GPRASP2蛋白的N端结构域，发现其与GPCR胞内结构域的相互作用模式，为靶向药物设计提供结构基础。
3. **Title**: "GPRASP2 promotes tumor metastasis via Wnt/β-catenin signaling"
**Authors**: Zhang L, et al.
**Summary**: 在乳腺癌模型中，重组GPRASP2过表达增强癌细胞侵袭能力，机制研究表明其通过调控β-catenin核转位激活促转移基因。
4. **Title**: "GPRASP2 deficiency exacerbates neurodegeneration in Alzheimer's models"
**Authors**: Lee C, et al.
**Summary**: 利用重组蛋白敲低技术，发现GPRASP2缺失加速淀粉样蛋白沉积，提示其在神经元内GPCR稳态维持中的保护作用。
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**注意**：以上内容为模拟生成的示例，实际研究需参考PubMed、Web of Science等平台的真实文献。建议使用关键词“GPRASP2”、“recombinant protein”或“GPCR sorting”进行检索。
GPRASP2 (G Protein-Coupled Receptor Associated Sorting Protein 2) is a member of the GASP family, known for its role in regulating the trafficking and degradation of G protein-coupled receptors (GPCRs). These receptors are critical for cellular signaling and mediate responses to neurotransmitters, hormones, and environmental stimuli. GPRASP2 interacts specifically with certain GPCRs, facilitating their lysosomal degradation and modulating receptor recycling, thereby influencing signal duration and cellular adaptation. It is implicated in neurological processes, including addiction and neurodevelopmental disorders, though its exact mechanisms remain under investigation.
Recombinant human GPRASP2 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional protein for in vitro studies. This tool enables researchers to explore GPCR regulatory pathways, protein interactions, and structural characteristics. Its applications span drug discovery, particularly in targeting GPCR-related diseases, and mechanistic studies of receptor downregulation. Current research focuses on elucidating its binding partners, tissue-specific roles, and potential therapeutic relevance in disorders linked to GPCR dysregulation. Its study contributes to broader insights into cellular signaling homeostasis and disease mechanisms.
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