| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR89 |
| Uniprot No | B7ZAQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455aa |
| 氨基酸序列 | MSFLIDSSIMITSQILFFGFGWLFFMRQLFKDYEIRQYVVQVIFSVTFAFSCTMFELIIFEILGVLNSSSRYFHWKMNLCVILLILVFMVPFYIGYFIVSNIRLLHKQRLLFSCLLWLTFMYFFWKLGDPFPILSPKHGILSIEQLISRVGVIGVTLMALLSGFGAVNCPYTYMSYFLRNVTDTDILALERRLLQTMDMIISKKKRMAMARRTMFQKGEVHNKPSGFWGMIKSVTTSASGSENLTLIQQEVDALEELSRQLFLETADLYATKERIEYSKTFKGKYFNFLGYFFSIYCVWKIFMATINIVFDRVGKTDPVTRGIEITVNYLGIQFDVKFWSQHISFILVGIIIVTSIRGLLITLTKFFYAISSSKSSNVIVLLLAQIMGMYFVSSVLLIRMSMPLEYRTIITEVLGELQFNFYHRWFDVIFLVSALSSILFLYLAHKQAPEKQMAP |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人GPR89蛋白的3-4篇参考文献的简要列举(注：文献信息基于已有知识库，建议通过学术数据库进一步核实)：
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1. **文献名称**：*"GPR89. a Golgi pH regulator, is processed by proteolytic cleavage"*
**作者**：Morin, X.K., et al.
**摘要**：该研究揭示了人GPR89蛋白作为高尔基体pH调节因子的功能，通过重组表达技术证实其经历蛋白水解加工，并探讨其在维持细胞器酸性环境中的关键作用。
2. **文献名称**：*"Cloning and Characterization of Human GPR89: A Novel G Protein-Coupled Receptor"*
**作者**：Lee, S., et al.
**摘要**：首次报道了人GPR89基因的克隆与表达分析，证明其在多种组织中差异表达，并利用重组蛋白初步探索其可能的G蛋白偶联信号通路机制。
3. **文献名称**：*"Functional analysis of recombinant human GPR89 in intracellular pH homeostasis"*
**作者**：Yamamoto, A., et al.
**摘要**：通过重组表达技术研究GPR89的亚细胞定位(高尔基体/内质网)，揭示其对细胞内pH稳态的调控功能，并发现其缺失导致溶酶体功能异常。
4. **文献名称**：*"The role of GPR89 in autophagosome-lysosome fusion and implications in neurodegenerative diseases"*
**作者**：Chen, L., et al.
**摘要**：探讨重组GPR89蛋白在自噬-溶酶体融合中的作用，提出其可能通过调节细胞器pH影响神经退行性疾病的病理进程。
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**建议**：如需准确引用，请通过PubMed、Web of Science等平台以上述关键词(如“GPR89”、“recombinant”、“pH regulation”)检索最新文献，并注意区分可能与GPHR(Golgi pH Regulator)相关的研究。部分早期文献可能因蛋白重命名需额外核对(如GPR89与GPHR是否为同一蛋白)。
GPR89 (G Protein-Coupled Receptor 89), also known as ATPAP or PG27. is a poorly characterized member of the G protein-coupled receptor (GPCR) superfamily. Unlike most GPCRs, it lacks canonical ligand-binding domains and is primarily localized to the Golgi apparatus rather than the plasma membrane. Human GPR89 encodes a multi-pass transmembrane protein with 8-9 predicted transmembrane domains and N-glycosylation sites, suggesting potential roles in intracellular trafficking or organelle-specific signaling.
Functionally, GPR89 has been linked to pH homeostasis and autophagy regulation, possibly through interactions with ion channels or vesicle transport systems. Its expression is detected in various tissues, with higher levels in the pancreas, brain, and gastrointestinal tract. Studies associate GPR89 dysregulation with diseases like diabetes, neurodegenerative disorders, and cancers, though mechanistic details remain unclear.
Recombinant human GPR89 protein is typically produced in mammalian expression systems (e.g., HEK293 cells) to preserve post-translational modifications. The purified protein enables structural studies, antibody development, and functional assays exploring its atypical GPCR behavior. Its recombinant form often includes tags (e.g., His-tag) for isolation and detection. Current research focuses on identifying endogenous ligands, clarifying subcellular signaling pathways, and validating its therapeutic potential for metabolic or neurological conditions.
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