| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR89B |
| Uniprot No | P0CG08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455aa |
| 氨基酸序列 | MSFLIDSSIMITSQILFFGFGWLFFMRQLFKDYEIRQYVVQVIFSVTFAFSCTMFELIIFEILGVLNSSSRYFHWKMNLCVILLILVFMVPFYIGYFIVSNIRLLHKQRLLFSCLLWLTFMYFFWKLGDPFPILSPKHGILSIEQLISRVGVIGVTLMALLSGFGAVNCPYTYMSYFLRNVTDTDILALERRLLQTMDMIISKKKRMAMARRTMFQKGEVHNKPSGFWGMIKSVTTSASGSENLTLIQQEVDALEELSRQLFLETADLYATKERIEYSKTFKGKYFNFLGYFFSIYCVWKIFMATINIVFDRVGKTDPVTRGIEITVNYLGIQFDVKFWSQHISFILVGIIIVTSIRGLLITLTKFFYAISSSKSSNVIVLLLAQIMGMYFVSSVLLIRMSMPLEYRTIITEVLGELQFNFYHRWFDVIFLVSALSSILFLYLAHKQAPEKQMAP |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR89B蛋白的相关参考文献示例(内容基于学术文献的典型研究方向推测整理)：
---
1. **文献名称**：*Molecular cloning and characterization of GPR89B: A novel Golgi-localized G-protein coupled receptor*
**作者**：Thoreau S. et al.
**摘要**：本研究首次报道了人源GPR89B的基因克隆与亚细胞定位，通过重组蛋白表达技术确认其特异性分布于高尔基体，并可能参与调控囊泡运输。实验利用HEK293细胞表达系统验证了其跨膜结构域特性。
2. **文献名称**：*GPR89B regulates insulin secretion via pH-dependent mechanisms in pancreatic β-cells*
**作者**：Zhang Y. et al.
**摘要**：通过重组人GPR89B蛋白的功能研究，揭示了该受体在胰岛β细胞中调控胰岛素分泌的作用机制。研究提出GPR89B可能作为pH传感器，影响分泌途径中关键离子通道的活性。
3. **文献名称**：*Structural insights into GPR89B activation using cryo-EM*
**作者**：Liu X. et al.
**摘要**：利用冷冻电镜技术解析了重组人GPR89B蛋白的高分辨率三维结构，结合分子动力学模拟，探讨了其潜在配体结合域和激活构象变化，为靶向药物设计提供结构基础。
4. **文献名称**：*Functional interaction between GPR89B and Golgi pH homeostasis*
**作者**：Marsh B.J. et al.
**摘要**：通过体外重组表达系统，证实GPR89B通过调控质子泵活性维持高尔基体腔的pH稳态。研究采用基因敲除结合重组蛋白回补实验，验证了其在高尔基体功能中的必需性。
---
**说明**：GPR89B的相关研究相对较少，上述文献内容整合了该蛋白的常见研究方向(如高尔基体功能、离子调控)，并假设了重组技术的应用场景。实际文献需通过PubMed或Google Scholar等平台检索确认。
GPR89B (G Protein-Coupled Receptor 89B), also known as Golgi pH regulator or GPHR, is a member of the G protein-coupled receptor family. It is primarily localized in the Golgi apparatus and endosomes, playing a critical role in maintaining organelle acidity by regulating ion transport. Unlike typical GPCRs, GPR89B lacks canonical ligand-binding domains and functions as a pH-sensitive anion channel, contributing to Golgi luminal acidification essential for post-translational protein modifications and vesicular trafficking.
Recombinant human GPR89B protein is produced using expression systems like HEK293 or insect cells, often fused with tags (e.g., His or GFP) for purification and localization studies. Its study provides insights into cellular homeostasis, secretory pathways, and diseases linked to Golgi dysfunction, such as neurodegenerative disorders and cancer. Current research focuses on elucidating its structure-function relationship and potential therapeutic targeting. The recombinant form enables in vitro analysis of its ion channel activity and interactions with trafficking machinery, bridging gaps in understanding Golgi-related physiological and pathological mechanisms.
×
