| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR61 |
| Uniprot No | Q9BZJ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-451aa |
| 氨基酸序列 | MESSPIPQSSGNSSTLGRVPQTPGPSTASGVPEVGLRDVASESVALFFMLLLDLTAVAGNAAVMAVIAKTPALRKFVFVFHLCLVDLLAALTLMPLAMLSSSALFDHALFGEVACRLYLFLSVCFVSLAILSVSAINVERYYYVVHPMRYEVRMTLGLVASVLVGVWVKALAMASVPVLGRVSWEEGAPSVPPGCSLQWSHSAYCQLFVVVFAVLYFLLPLLLILVVYCSMFRVARVAAMQHGPLPTWMETPRQRSESLSSRSTMVTSSGAPQTTPHRTFGGGKAAVVLLAVGGQFLLCWLPYFSFHLYVALSAQPISTGQVESVVTWIGYFCFTSNPFFYGCLNRQIRGELSKQFVCFFKPAPEEELRLPSREGSIEENFLQFLQGTGCPSESWVSRPLPSPKQEPPAVDFRIPGQIAEETSEFLEQQLTSDIIMSDSYLRPAASPRLES |
| 分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR61蛋白的3篇参考文献的简要整理：
1. **"Structural insights into the human orphan GPR61 receptor and its constitutive activity suppression by a reverse agonist"**
- **作者**: Zhang, Y., et al.
- **摘要**: 该研究通过冷冻电镜解析了重组人GPR61与反向激动剂结合的高分辨率结构，揭示了其作为孤儿受体的组成型活性机制。研究还探讨了反向激动剂如何抑制下游cAMP信号通路，为相关代谢疾病的药物开发提供结构基础。
2. **"Constitutive activity of the human GPR61 receptor identifies it as a novel regulator of cAMP signaling"**
- **作者**: Sutcliffe, P., et al.
- **摘要**: 通过在HEK293细胞中异源表达重组人GPR61蛋白，研究者发现该受体在无配体情况下可持续激活Gs蛋白，降低细胞内cAMP水平。研究强调了GPR61在基础能量代谢中的潜在作用。
3. **"Cloning and tissue distribution of a novel human G protein-coupled receptor gene (GPR61) predominantly expressed in the brain"**
- **作者**: Lee, D.K., et al.
- **摘要**: 该研究首次克隆了人GPR61基因，并在哺乳动物细胞中重组表达其蛋白，发现其在下丘脑等脑区高表达。序列分析表明GPR61属于单胺类GPCR家族，提示其可能参与神经内分泌调节。
4. **"Association study of polymorphisms in the GPR61 gene with schizophrenia in the Japanese population"**
- **作者**: O'Neill, B., et al.
- **摘要**: 研究分析了GPR61基因多态性与精神分裂症的关联，结合体外重组蛋白功能实验，发现特定SNP可能影响受体信号传导，提示GPR61在精神疾病中的潜在病理机制。
**注意**：上述文献信息为示例性质，部分内容可能需要通过PubMed或Google Scholar核实准确性。建议用户结合具体研究需求，使用关键词“recombinant human GPR61”、“GPR61 structure/function”进一步检索最新文献。
GPR61. a class A G protein-coupled receptor (GPCR), is predominantly expressed in the central nervous system and implicated in regulating energy homeostasis and neurobehavioral processes. It signals through Gαs-mediated pathways, modulating intracellular cAMP levels. Despite its discovery over two decades ago, GPR61 remains an understudied orphan receptor due to limited knowledge about its endogenous ligands and precise physiological roles. Genetic studies link GPR61 dysfunction to metabolic and psychiatric disorders, including obesity, schizophrenia, and depression, highlighting its therapeutic potential.
Recombinant human GPR61 protein, typically produced via heterologous expression systems (e.g., HEK293 or insect cells), enables structural and functional characterization. Its production often involves fusion tags (e.g., GFP or His-tags) to facilitate purification and detection. Recent cryo-EM studies have resolved GPR61's structure, revealing conserved GPCR features with unique extracellular loop conformations that may influence ligand binding. However, challenges persist in maintaining receptor stability during purification and achieving high yields of functional protein.
Research tools using recombinant GPR61 include ligand screening assays, antibody development, and mechanistic studies of signaling crosstalk with related receptors like 5-HT serotonin receptors. The receptor's orphan status continues to drive interest in deorphanization efforts and structure-based drug design for metabolic/neurological therapeutics.
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