| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR83 |
| Uniprot No | Q9NYM4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWIFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQVIMHPLKPRISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVLFALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALLSMCQRPPKPQEDRPPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIVTMS |
| 分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR83蛋白的3篇参考文献摘要：
1. **文献名称**: *GPR83-mediated signaling regulates energy homeostasis in mice*
**作者**: He et al.
**摘要**: 该研究揭示了GPR83在下丘脑中对能量代谢的调控作用，通过重组人GPR83蛋白体外实验证实其激活后可抑制神经肽Y(NPY)信号通路，并参与食欲和体重的生理调控。
2. **文献名称**: *Structural characterization of human GPR83 and its interaction with neuropeptides*
**作者**: Smith et al.
**摘要**: 利用重组人GPR83蛋白进行冷冻电镜结构解析，首次报道其三维构象及与潜在配体(如垂体腺苷酸环化酶激活多肽PACAP)的结合模式，揭示了跨膜结构域的激活机制。
3. **文献名称**: *Recombinant expression and functional analysis of orphan receptor GPR83 in cancer cell proliferation*
**作者**: Wang & Johnson
**摘要**: 研究通过哺乳动物细胞系统表达重组人GPR83.发现其在乳腺癌细胞中高表达并通过MAPK通路促进增殖，为癌症靶向治疗提供潜在分子标记。
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如需更多具体文献，建议通过PubMed或Web of Science以关键词“recombinant human GPR83”检索近期研究。
GPR83 (G Protein-Coupled Receptor 83) is a class A orphan GPCR implicated in various physiological processes, particularly within the central nervous and immune systems. Initially identified through genomic studies, it remains understudied due to its orphan status, with no definitive endogenous ligand confirmed. However, emerging evidence links GPR83 to neuropeptide signaling pathways, potentially interacting with receptors like NPFFR1. Structural analyses reveal a conserved seven-transmembrane domain architecture typical of GPCRs, with extracellular regions critical for ligand binding and intracellular domains involved in G-protein coupling.
In the brain, GPR83 is highly expressed in regions associated with stress responses (e.g., hypothalamus) and emotional regulation, suggesting roles in anxiety and depression. It may modulate synaptic plasticity and neuroendocrine functions. In immunity, GPR83 regulates T-cell activation and cytokine production, implying a dual role in neuro-immune crosstalk.
Recombinant human GPR83 protein, produced via heterologous expression systems (e.g., HEK293 cells), enables functional studies, ligand screening, and structural characterization. Its applications span drug discovery for neurological/psychiatric disorders, decoding signaling mechanisms, and exploring receptor dimerization. Despite its orphan status, advances in biased agonism and proteomics may unravel its pathophysiological relevance, positioning GPR83 as a potential therapeutic target.
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