| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPRIN2 |
| Uniprot No | O60269 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MSSSHPEPGPWAPLSPRLQPLSQSSSSLLGEGREQRPELHKTASSTMWQAQLGEASTRPQAPEEEGNPPESMKPARASGPKARPSAGGHWRSSTVGNVSTMGGGDLCRLRAPSAAAMQRSHSDLVRSTQMRGHSGARKASLSCSALGSSPVHRAQLQPGGTSGQGGQAPAGLERDLAPEDETSNSAWMLGASQLSVPPLDLGDTTAHSSSAQAEPKAAEQLATTTCHALPPAALLCGMREMREVGAGGCCHALPATGILAFPKLVASVSESGLQAQHGVKIHCRLSGGLPGHSHCCAHLWGPAGLVPEPGSRTKDVWTMTSANDLAPAEASPLSAQDAGVQAAPVAACKAVATSPSLEAPAALHVFPEVTLGSSLEEAPSPVRDVRWDAEGMTWEVYGAAVDPEVLGVAIQKHLEMQFEQLQRAPASEDSLSVEGRRGPLRAVMQSLRRPSCCGCSGAAPE |
| 分子量 | 74.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPRIN2蛋白的示例性参考文献(注：以下内容为模拟文献概要，建议通过学术数据库查询真实文献)：
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1. **文献名称**：*Expression and Purification of Recombinant Human GPRIN2 in Escherichia coli*
**作者**：Zhang Y, et al.
**摘要**：研究报道了利用大肠杆菌表达系统高效表达重组人GPRIN2蛋白的优化方法，通过His标签亲和层析纯化获得高纯度蛋白，并验证其稳定性，为后续功能研究提供基础材料。
2. **文献名称**：*GPRIN2 Regulates Neuronal Differentiation via cAMP/PKA Signaling Pathway*
**作者**：Li X, et al.
**摘要**：探讨重组人GPRIN2蛋白在神经细胞分化中的作用，发现其通过调控cAMP/PKA信号通路促进神经元突触形成，为神经发育相关疾病机制提供新视角。
3. **文献名称**：*Structural Characterization of Recombinant GPRIN2 and Its Interaction with G Proteins*
**作者**：Chen J, et al.
**摘要**：利用圆二色谱和表面等离子共振技术解析重组GPRIN2的二级结构，并证实其与G蛋白βγ亚基的相互作用，揭示其在跨膜信号转导中的潜在功能。
4. **文献名称**：*GPRIN2 Modulates Oxidative Stress Response in Neurodegenerative Models*
**作者**：Wang H, et al.
**摘要**：研究重组人GPRIN2蛋白在帕金森病细胞模型中的神经保护作用，发现其通过降低ROS水平减轻氧化应激损伤，提示其作为治疗靶点的可能性。
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建议通过 **PubMed** 或 **Web of Science** 搜索关键词“recombinant human GPRIN2”获取真实文献。如需具体文献指导，请提供更详细的研究方向(如表达方法或功能分析)。
GPRIN2 (G Protein-Regulated Inducer of Neuronal regeneration 2) is a human protein encoded by the *GPRIN2* gene, located on chromosome 4q32.3. It belongs to the GPRIN family, which is implicated in neuronal development, regeneration, and synaptic plasticity. Structurally, GPRIN2 contains conserved N-terminal transmembrane domains and a C-terminal region with potential phosphorylation sites, suggesting roles in signal transduction. Studies indicate it interacts with G-protein-coupled receptors (GPCRs) and modulates pathways linked to neurite outgrowth, neuronal survival, and neurotransmitter release. Its involvement in NMDA receptor signaling and BDNF (brain-derived neurotrophic factor) pathways highlights its significance in neuropsychiatric and neurodegenerative disorders.
Recombinant human GPRIN2 protein is synthesized using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purification often involves affinity tags like His-tag for isolation. This engineered protein enables researchers to explore GPRIN2’s biochemical properties, receptor interactions, and downstream signaling *in vitro* or *in vivo*. Current research focuses on its potential as a therapeutic target for Alzheimer’s disease, Parkinson’s disease, and depression, given its regulatory roles in neuroprotection and synaptic plasticity. However, its precise molecular mechanisms remain under investigation, necessitating further structural and functional analyses.
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