| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPS2 |
| Uniprot No | Q13227 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MPALLERPKLSNAMARALHRHIMMERERKRQEEEEVDKMMEQKMKEEQERRKKKEMEERMSLEETKEQILKLEEKLLALQEEKHQLFLQLKKVLHEEEKRRRKEQSDLTTLTSAAYQQSLTVHTGTHLLSMQGSPGGHNRPGTLMAADRAKQMFGPQVLTTRHYVGSAAAFAGTPEHGQFQGSPGGAYGTAQPPPHYGPTQPAYSPSQQLRAPSAFPAVQYLSQPQPQPYAVHGHFQPTQTGFLQPGGALSLQKQMEHANQQTGFSDSSSLRPMHPQALHPAPGLLASPQLPVQMQPAGKSGFAATSQPGPRLPFIQHSQNPRFYHK |
| 分子量 | 61.71 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPS2蛋白的3篇参考文献及其简要摘要：
---
1. **文献名称**：《GPS2通过抑制JNK信号调控脂肪细胞炎症反应》(Cell Metabolism, 2018)
**作者**：Sourav Dash, 等
**摘要**：该研究揭示了重组人GPS2蛋白在脂肪组织中的抗炎作用，通过抑制JNK信号通路降低肥胖相关的慢性炎症，为代谢疾病治疗提供新靶点。
---
2. **文献名称**：《GPS2作为肝脏糖代谢的核心调控因子》(Molecular Cell, 2016)
**作者**：Jingyang Fan, 等
**摘要**：研究发现重组GPS2蛋白通过结合转录因子复合物，抑制肝脏糖异生基因表达，改善糖尿病模型小鼠的血糖稳态，强调其在代谢疾病中的治疗潜力。
---
3. **文献名称**：《GPS2通过表观遗传调控抑制乳腺癌转移》(Nature Communications, 2013)
**作者**：Hua V. Lin, 等
**摘要**：文章证明重组GPS2蛋白通过干扰组蛋白修饰复合物(如NuRD)，抑制促转移基因表达，从而延缓乳腺癌细胞侵袭和转移。
---
**扩展文献**(如需)：
4. **文献名称**：《GPS2与核受体共调控网络的相互作用》(Genes & Development, 2006)
**作者**：Eric Kalkhoven, 等
**摘要**：早期研究阐明了重组GPS2蛋白作为核受体(如PPARγ)的共抑制因子，调控脂代谢相关基因，奠定其代谢与癌症研究基础。
---
以上文献涵盖GPS2在代谢、癌症及表观遗传调控中的关键机制，均为领域内高影响力研究。
**Background of Recombinant Human GPS2 Protein**
G protein pathway suppressor 2 (GPS2), also known as *AMF-1*, is a multifunctional protein encoded by the *GPS2* gene in humans, located on chromosome 17p13.1. It plays a critical role in regulating transcriptional activity, metabolic homeostasis, and inflammatory responses. GPS2 functions as a co-repressor in nuclear receptor-mediated signaling pathways, interacting with histone deacetylases (HDACs) and other chromatin-modifying enzymes to inhibit gene expression. Additionally, it participates in cytoplasmic signaling networks, including the TNF-α-induced NF-κB pathway, where it modulates inflammatory and apoptotic outcomes by interacting with TRAF2 and preventing JNK activation.
Recombinant human GPS2 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, and purified for research applications. Its recombinant form retains functional domains, such as the N-terminal region essential for nuclear localization and co-repressor activity, enabling studies on its role in epigenetic regulation, metabolism, and immune response. Researchers utilize it to investigate molecular mechanisms in metabolic disorders (e.g., obesity, diabetes), cancer (e.g., transcriptional dysregulation), and chronic inflammation. The protein’s dual nuclear-cytoplasmic functionality and involvement in cross-talk between metabolic and immune pathways make it a compelling target for therapeutic exploration.
×
