| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPS1 |
| Uniprot No | Q13098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475aa |
| 氨基酸序列 | MQIDVDPQEDPQNAPDVNYVVENPSLDLEQYAASYSGLMRIERLQFIADHCPTLRVEALKMALSFVQRTFNVDMYEEIHRKLSEATRSSLRELQNAPDAIPESGVEPPALDTAWVEATRKKALLKLEKLDTDLKNYKGNSIKESIRRGHDDLGDHYLDCGDLSNALKCYSRARDYCTSAKHVINMCLNVIKVSVYLQNWSHVLSYVSKAESTPEIAEQRGERDSQTQAILTKLKCAAGLAELAARKYKQAAKCLLLASFDHCDFPELLSPSNVAIYGGLCALATFDRQELQRNVISSSSFKLFLELEPQVRDIIFKFYESKYASCLKMLDEMKDNLLLDMYLAPHVRTLYTQIRNRALIQYFSPYVSADMHRMAAAFNTTVAALEDELTQLILEGLISARVDSHSKILYARDVDQRSTTFEKSLLMGKEFQRRAKAMMLRAAVLRNQIHVKSPPREGSQGELTPANSQSRMSTNM |
| 分子量 | 77.88 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPS1蛋白的**模拟参考文献示例**(请注意，这些文献为虚拟示例，实际研究请通过学术数据库核实)：
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1. **文献名称**：*Structural Insight into Human GPS1/COPS1 in the COP9 Signalosome Complex*
**作者**：Li Y, et al.
**摘要**：本研究通过重组表达人GPS1蛋白，解析了其在COP9信号复合体中的三维结构，揭示了其对Cullin-RING泛素连接酶去NEDD化的调控机制，为靶向泛素-蛋白酶体系统的药物开发提供依据。
2. **文献名称**：*Recombinant GPS1 Modulates JNK Signaling in Cancer Cell Apoptosis*
**作者**：Wang X, et al.
**摘要**：利用昆虫细胞系统表达重组人GPS1蛋白，发现其通过抑制JNK磷酸化通路增强化疗药物诱导的肿瘤细胞凋亡，提示GPS1在癌症治疗中的潜在应用价值。
3. **文献名称**：*GPS1-Mediated NF-κB Inhibition via IκB Stabilization in Macrophages*
**作者**：Kim S, et al.
**摘要**：通过体外重组GPS1蛋白的功能实验，证明其通过结合IKKγ(NEMO)抑制IκBα降解，从而阻断NF-κB炎症信号传导，为抗炎治疗提供新靶点。
4. **文献名称**：*Expression and Functional Characterization of Human GPS1 in Arabidopsis*
**作者**：Martinez R, et al.
**摘要**：在植物模型中异源表达重组人GPS1蛋白，发现其能够调控光形态建成相关基因，表明GPS1在进化中保守的蛋白修饰功能。
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**建议**：如需真实文献，建议在PubMed、Google Scholar等平台以“GPS1”、“COPS1”、“COP9 signalosome”及“recombinant protein”为关键词检索，并结合近五年研究筛选。
Recombinant human GPS1 (G Protein Pathway Suppressor 1) protein, also known as COP9 signalosome subunit 1 (CSN1) or JAB1-containing signalosome subunit 1. is a critical component of the COP9 signalosome (CSN) complex. This evolutionarily conserved multiprotein complex regulates ubiquitin-proteasome-mediated protein degradation by interacting with cullin-RING E3 ubiquitin ligases (CRLs), modulating their activity through deneddylation. GPS1 plays a central role in maintaining CSN structural integrity and mediating substrate recognition.
Studies highlight GPS1's involvement in diverse cellular processes, including DNA repair, cell cycle control, and signal transduction pathways such as NF-κB and p53. Its dysfunction has been linked to cancer progression, immune disorders, and developmental defects. Recombinant GPS1 proteins, typically expressed in *E. coli* or mammalian systems, retain post-translational modification capacities when properly folded. These proteins are invaluable tools for biochemical studies—such as protein-protein interaction assays, enzymatic activity analyses, and structural biology investigations—to dissect the COP9 signalosome's regulatory mechanisms.
Recent research focuses on GPS1's potential as a therapeutic target, particularly in cancers with altered ubiquitination pathways. Engineered variants (e.g., mutant or tagged forms) further enable targeted studies of its molecular interactions. As a key orchestrator of protein homeostasis, GPS1 remains pivotal in understanding cellular adaptation to stress and disease pathogenesis.
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