| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPSM3 |
| Uniprot No | Q9Y4H4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160aa |
| 氨基酸序列 | MEAERPQEEEDGEQGPPQDEEGWPPPNSTTRPWRSAPPSPPPPGTRHTALGPRSASLLSLQTELLLDLVAEAQSRRLEEQRATFYTPQNPSSLAPAPLRPLEDREQLYSTILSHQCQRMEAQRSEPPLPPGGQELLELLLRVQGGGRMEEQRSRPPTHTC |
| 分子量 | 43.34 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPSM3蛋白的3篇参考文献概述(注：因文献检索限制，以下为模拟示例，实际文献需通过学术数据库查询)：
1. **文献名称**: "GPSM3 regulates the immune response via modulation of G-protein signaling in macrophages"
**作者**: Zhang L, et al.
**摘要**: 研究探讨了重组人GPSM3蛋白在巨噬细胞中的免疫调节功能，发现其通过抑制Gαi蛋白信号通路，降低炎症因子IL-6和TNF-α的表达，揭示了其在自身免疫疾病中的潜在治疗价值。
2. **文献名称**: "Structural characterization of recombinant human GPSM3 and its role in cell migration"
**作者**: Chen Y, et al.
**摘要**: 通过X射线晶体学解析了重组GPSM3蛋白的3D结构，发现其含有典型GoLoco结构域，并证明其通过结合Gαi亚基调控细胞骨架重排，影响癌细胞迁移能力。
3. **文献名称**: "GPSM3 deficiency exacerbates inflammatory arthritis through enhanced NLRP3 inflammasome activation"
**作者**: Wang H, et al.
**摘要**: 在小鼠模型中发现GPSM3缺失会导致NLRP3炎症小体过度激活，而过表达重组人GPSM3蛋白可显著缓解关节炎症，提示其在炎症性关节炎中的保护作用。
建议通过PubMed或Google Scholar以关键词 **"recombinant GPSM3" / "human GPSM3 function"** 获取更多最新文献。实际研究中GPSM3常与G蛋白信号传导及免疫调控相关。
GPSM3 (G-protein signaling modulator 3), also known as activator of G-protein signaling 3 (AGS3), is a cytosolic protein involved in regulating G-protein-coupled receptor (GPCR) signaling pathways. It belongs to the GPSM/LGN family, characterized by conserved tetratricopeptide (TPR) and GoLoco motifs. The GoLoco motifs enable GPSM3 to bind inactive Gαi subunits, stabilizing their GDP-bound state and inhibiting G protein signaling. This interaction influences cellular processes like cell division, polarization, and migration.
Research highlights GPSM3’s role in immune regulation, particularly in hematopoietic cells. It modulates T-cell receptor signaling, dendritic cell maturation, and mast cell activation, linking it to inflammatory and allergic responses. Genetic studies associate GPSM3 polymorphisms with autoimmune diseases (e.g., rheumatoid arthritis) and cancer susceptibility, suggesting its dual roles in immune homeostasis and oncogenesis.
Recombinant human GPSM3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, interaction partners, and signaling mechanisms *in vitro*. Its production enables therapeutic exploration, such as targeting dysregulated GPCR pathways in immune disorders or cancer. Current studies focus on elucidating its regulatory networks and validating its potential as a biomarker or drug target, emphasizing its emerging significance in precision medicine and molecular pharmacology.
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