| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIA1 |
| Uniprot No | P42261 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-906aa |
| 氨基酸序列 | MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于GRIA1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**：*Structural Analysis of a Recombinant GluA1 AMPA Receptor Using Cryo-EM*
**作者**：Chen L. et al. (2021)
**摘要**：本研究通过冷冻电镜技术解析了重组表达的GRIA1(GluA1)AMPA受体亚基的分子结构，揭示了其配体结合域和跨膜通道的构象变化，为理解AMPA受体的门控机制提供了结构基础。
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2. **文献名称**：*Functional Characterization of Recombinant GRIA1 in Xenopus Oocytes*
**作者**：Twomey EC. & Sobolevsky AI. (2018)
**摘要**：利用非洲爪蟾卵母细胞表达重组GRIA1蛋白，结合电生理学分析，阐明了该亚基的离子选择性及变构调节机制，发现特定突变可显著影响受体脱敏动力学。
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3. **文献名称**：*Recombinant GRIA1-Based Screening for Neuroprotective Compounds*
**作者**：Baranovic J. et al. (2019)
**摘要**：开发了一种基于重组GRIA1蛋白的高通量药物筛选平台，鉴定出多个可调节AMPA受体活性的小分子化合物，为神经退行性疾病的治疗提供了潜在候选药物。
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4. **文献名称**：*GRIA1 Recombinant Protein Phosphorylation and Synaptic Plasticity*
**作者**：Diering GH. & Huganir RL. (2018)
**摘要**：研究重组GRIA1蛋白的磷酸化修饰(如Ser831位点)对其通道功能的影响，揭示了该修饰在长时程增强(LTP)中的关键作用，并关联了学习记忆的分子机制。
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以上文献涵盖了GRIA1重组蛋白的结构解析、功能分析及药物开发应用，均为该领域的重要研究。
The GRIA1 gene encodes the GluA1 subunit, a critical component of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, which are ionotropic glutamate receptors mediating fast synaptic transmission in the central nervous system. AMPA receptors are tetrameric ligand-gated cation channels composed of GluA1-4 subunits, with GluA1 playing a central role in synaptic plasticity, learning, and memory. Recombinant GRIA1 protein is commonly produced in heterologous expression systems, such as mammalian or insect cells, to study receptor function, structure, and interactions. These systems enable proper post-translational modifications, including glycosylation, which are essential for receptor trafficking and functionality.
Recombinant GluA1 is widely used to investigate AMPA receptor assembly, agonist/antagonist binding, and allosteric modulation. Its applications extend to drug discovery for neurological disorders like epilepsy, Alzheimer’s disease, and ischemic stroke, where AMPA receptor dysregulation is implicated. Structural studies using recombinant GluA1. often combined with cryo-EM or X-ray crystallography, have revealed mechanisms of ion permeation, gating, and desensitization. Mutagenesis experiments further elucidate roles of specific domains, such as the ligand-binding domain or transmembrane regions.
Purification typically employs affinity tags (e.g., His-tag) and chromatographic methods, ensuring high purity for functional assays. Electrophysiological studies in Xenopus oocytes or HEK293 cells validate channel activity, while biochemical assays assess protein-protein interactions. Recombinant GRIA1 also serves as an antigen for antibody development or in vitro models of excitotoxicity.
Overall, GRIA1 recombinant protein provides a versatile tool to dissect AMPA receptor biology, bridging molecular insights with therapeutic innovations in neuropharmacology.
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