| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACF |
| Uniprot No | Q9NRL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1415-1545aa |
| 氨基酸序列 | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEPSPVTLGRRSSGRQGGVH ELSAFEQLVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKPIALNIIREKV NKCEYKLASEFIDDIELMFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQKL GLHVTPSNVDQV |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACF(APOBEC1 Complementation Factor)重组蛋白的3篇参考文献示例(文献为示例性概括，非真实存在)：
1. **文献名称**：*Expression and Purification of Recombinant ACF for RNA Editing Studies*
**作者**：Smith, J.R. et al.
**摘要**：该研究报道了在大肠杆菌中高效表达并纯化重组ACF蛋白的方法，验证了其与APOBEC1协同作用促进RNA编辑的功能，为体外编辑机制研究提供了工具。
2. **文献名称**：*Structural Insights into ACF’s Role in APOBEC1-Mediated mRNA Editing*
**作者**：Chen, L. & Watanabe, M.
**摘要**：通过X射线晶体学解析了ACF与APOBEC1的复合物结构，揭示了ACF通过结合RNA和APOBEC1调控靶标mRNA特异性的分子机制。
3. **文献名称**：*Functional Characterization of Recombinant ACF in Hyperlipidemia Models*
**作者**：Guo, Y. et al.
**摘要**：利用重组ACF蛋白在小鼠模型中恢复APOBEC1/ACF复合物的活性，证实其通过编辑ApoB mRNA改善脂代谢紊乱的潜在治疗价值。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“ACF recombinant protein”或“APOBEC1 complementation factor”获取最新研究。
**Background of ACF Recombinant Protein**
The ATP-utilizing chromatin assembly and remodeling factor (ACF) is a multi-subunit protein complex critical for chromatin organization and gene regulation. Initially identified in *Drosophila melanogaster*, ACF belongs to the ISWI (Imitation Switch) family of chromatin remodelers, which regulate DNA accessibility by altering nucleosome positioning. The canonical ACF complex consists of two core subunits: the ATPase ISWI (SNF2L in humans) and Acf1 (BAZ1A in humans), a scaffolding protein essential for substrate recognition and complex stability.
ACF plays a pivotal role in *de novo* chromatin assembly, nucleosome spacing, and transcriptional repression. By harnessing energy from ATP hydrolysis, it slides nucleosomes along DNA to establish ordered chromatin structures, thereby influencing processes like DNA replication, repair, and gene expression. Its activity is tightly regulated by post-translational modifications and interactions with histone chaperones or other chromatin-associated factors.
Recombinant ACF proteins are produced via heterologous expression systems (e.g., *E. coli* or insect cells) to study its biochemical and structural properties. These recombinant forms retain ATPase activity and nucleosome remodeling capabilities, enabling *in vitro* investigations into chromatin dynamics. Researchers utilize purified ACF to dissect mechanisms of nucleosome positioning, histone exchange, and epigenetic regulation.
The development of recombinant ACF has advanced our understanding of chromatin biology, offering insights into diseases linked to chromatin dysregulation, such as cancer and developmental disorders. Studies leveraging recombinant ACF also contribute to drug discovery, particularly in targeting chromatin remodelers for therapeutic intervention. Overall, ACF recombinant proteins serve as indispensable tools for exploring the molecular basis of genome organization and function.
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