| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDX |
| Uniprot No | Q7Z353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690aa |
| 氨基酸序列 | MNLRSVFTVEQQRILQRYYENGMTNQSKNCFQLILQCAQETKLDFSVVRTWVGNKRRKMSSKNSESGTATTGTSLSAPDITVRNVVNIARPSSQQSSWTSANNDVIVTGIYSPASSSSRQGTNKHTDTQITEAHKIPIQKTATKNDTEFQLHIPVQRQVAHCKNASLLLGEKTIILSRQTSVLNAGNSVFNHAKKNYGNSSVQASEMTVPQKPSVCHRPCKIEPVGIQRSYKPEHTGPALHNLCGQKPTIRDPYCRTQNLEIREVFSLAVSDYPQRILGGNAPQKPSSAEGNCLSIAMETGDAEDEYAREEELASMRAQIPSYSRFYESGSSLRAENQSTTLPGPGRNMPNSQMVNIRDMSDNVLYQNRNYHLTPRTSLHTASSTMYSNTNPLRSNFSPHFASSNQLRLSQNQNNYQISGNLTVPWITGCSRKRALQDRTQFSDRDLATLKKYWDNGMTSLGSVCREKIEAVATELNVDCEIVRTWIGNRRRKYRLMGIEVPPPRGGPADFSEQPESGSLSALTPGEEAGPEVGEDNDRNDEVSICLSEGSSQEEPNEVVPNDARAHKEEDHHAVTTDNVKIEIIDDEESDMISNSEVEQVNSFLDYKNEEVKFIENELEIQKQKYFKLQTFVRSLILAMKADDKEQQQALLSDLPPELEEMDFNHASLEPDDTSFSVSSLSEKNVSESL |
| 分子量 | 103.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为围绕重组人HDX蛋白的3篇虚构参考文献示例(请注意，作者及文献名为假设性内容，仅供格式参考)：
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1. **文献名称**：*Expression and Purification of Recombinant Human HDX Protein in E. coli for Structural Studies*
**作者**：Chen L. et al.
**摘要**：本研究成功在大肠杆菌系统中表达了重组人HDX蛋白，并通过镍柱亲和层析和凝胶过滤层析实现了高纯度制备。实验表明，优化后的表达条件可显著提高蛋白可溶性，为后续HDX蛋白的结晶及功能分析奠定基础。
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2. **文献名称**：*Crystal Structure Analysis of Human HDX Protein Reveals Potential Drug-Binding Pockets*
**作者**：Zhang Y. et al.
**摘要**：通过X射线晶体学解析了重组人HDX蛋白的3.2Å分辨率三维结构，发现其N端结构域含有保守的ATP结合基序，且C端存在独特的疏水空腔。分子对接实验提示该区域可能成为小分子抑制剂的设计靶点，为癌症治疗提供新方向。
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3. **文献名称**：*Functional Characterization of Recombinant HDX Protein in Neuronal Apoptosis Regulation*
**作者**：Martinez R. et al.
**摘要**：体外实验证实重组人HDX蛋白可通过与Bcl-2家族蛋白相互作用调控线粒体膜电位，抑制氧化应激诱导的神经元凋亡。在小鼠模型中，HDX过表达显著减少缺血再灌注损伤后的脑细胞死亡，提示其在神经退行性疾病中的潜在治疗价值。
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**备注**：若“HDX”指代特定蛋白(如组蛋白去乙酰化酶相关或氢氘交换技术)，文献方向会有所调整。建议结合具体领域数据库(如PubMed)以“recombinant human HDX protein”为关键词检索真实文献。
Recombinant human HDX protein, a genetically engineered form of the human HDX protein, is produced in vitro using expression systems like bacteria, yeast, or mammalian cells. HDX (Hypothetical DUF-x protein) belongs to the functionally enigmatic "Domain of Unknown Function" (DUF) family, often implicated in cellular processes such as signaling, metabolism, or stress responses. While its exact biological role remains under investigation, HDX is hypothesized to interact with nucleic acids or other proteins based on structural predictions. Recombinant production enables large-scale purification for functional and structural studies, overcoming challenges like low natural abundance. Researchers utilize it to explore HDX’s involvement in diseases, such as cancer or neurodegenerative disorders, via knock-down/overexpression experiments. Techniques like X-ray crystallography, cryo-EM, or biochemical assays rely on recombinant HDX to decode its molecular mechanisms. Additionally, it serves as an antigen for antibody development or a control in diagnostic assays. Advances in protein engineering, including tagging (e.g., His-tag) or site-directed mutagenesis, further enhance its utility in mechanistic studies. This tool bridges gaps in understanding poorly characterized proteins, accelerating translational research in biomedicine.
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