| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEPN1 |
| Uniprot No | Q6WQI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MGNWGLGIAPWVDGESELEFRRLGMQGPLEALRRREWNTQRASFSFSFLIALSPHTVDYCHSYELFNRRWHGHVLATQRPSLFILMLV |
| 分子量 | 36.08 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HEPN1蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**: *HEPN1 Suppresses Hepatocellular Carcinoma Growth by Regulating Cell Cycle and Apoptosis*
**作者**: Zhang Y. et al.
**摘要**: 本研究利用重组人HEPN1蛋白，通过体外和体内实验证明其能够抑制肝癌细胞增殖。结果表明，HEPN1通过调控细胞周期蛋白(如Cyclin D1)并激活caspase依赖性凋亡通路发挥抗肿瘤作用，为肝癌治疗提供了潜在靶点。
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2. **文献名称**: *Recombinant HEPN1 Protein Exhibits Antibacterial Activity via RNA Cleavage*
**作者**: Li T. et al.
**摘要**: 本文报道了重组HEPN1蛋白的纯化及其抗菌机制。实验发现HEPN1通过与细菌毒素-抗毒素系统中的同源蛋白互作，发挥核糖核酸酶活性，特异性切割细菌RNA，提示其在对抗耐药菌感染中的潜在应用。
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3. **文献名称**: *Structural Insights into HEPN1-Mediated Viral RNA Recognition*
**作者**: Wang X. et al.
**摘要**: 本研究通过X射线晶体学解析了重组HEPN1蛋白的晶体结构，揭示其与病毒RNA结合的关键结构域。功能实验进一步证明，HEPN1通过靶向RNA二级结构抑制病毒复制，为开发广谱抗病毒药物奠定基础。
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注：以上文献为示例，具体研究需根据实际发表的论文补充作者和细节。建议通过PubMed或Google Scholar以“recombinant HEPN1 protein”为关键词检索最新成果。
Recombinant human HEPN1 (Hepatocellular Carcinoma, Novel1) protein is a biologically engineered version of the HEPN1 protein encoded by the *HEPN1* gene. This gene, located on chromosome 11 in humans, has garnered attention due to its dual role in cellular processes and disease pathogenesis. HEPN1 contains a conserved HEPN (Higher Eukaryotes and Prokaryotes Nucleotide-binding) domain, found in proteins associated with RNA metabolism and stress responses. Studies suggest HEPN1 participates in regulating apoptosis, cell proliferation, and mitochondrial function, though its exact molecular mechanisms remain under investigation. It is proposed to act as a tumor suppressor in hepatocellular carcinoma (HCC) by modulating the intrinsic apoptosis pathway via interactions with Bcl-2 family proteins. However, conflicting reports highlight context-dependent roles, with some evidence implicating HEPN1 in promoting tumor progression under specific conditions. Recombinant HEPN1 is typically produced in *E. coli* or mammalian expression systems, enabling functional studies such as protein-protein interaction assays, *in vitro* apoptosis models, and therapeutic exploration. Its application spans basic research—elucidating HEPN1’s physiological functions—to translational studies targeting HCC and other cancers. Despite progress, challenges persist in resolving contradictory findings and defining HEPN1’s tissue-specific signaling networks, underscoring the need for further mechanistic characterization.
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