| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEL308 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 275-337aa |
| 氨基酸序列 | GNAKAQTPIFSRSKQLKDTLLSEEINVAKKTIESSSNDLGPFYSLPSKVRDLYAQFKGIEKLY |
| 分子量 | 32.67 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HEL308蛋白的3条参考文献及其摘要概括：
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1. **文献名称**: *HEL308 suppresses homologous recombination and promotes a cellular response to replication fork stress*
**作者**: S. Tarsounas et al.
**摘要**: 研究揭示HEL308通过抑制同源重组修复(HR)维持DNA复制叉稳定性。重组人HEL308蛋白实验显示其解旋酶活性在复制应激时保护停滞的复制叉，并参与调控RAD51依赖性重组。
2. **文献名称**: *The DNA helicase HEL308 facilitates repair of DNA interstrand crosslinks*
**作者**: M. D’Alessandro et al.
**摘要**: 该文发现重组人HEL308蛋白通过促进DNA链间交联(ICL)的修复，维持基因组稳定性。其通过调控Fanconi贫血通路和复制叉重启机制发挥作用，提示其在癌症治疗中的潜在靶点价值。
3. **文献名称**: *Structural and functional characterization of the human helicase HEL308*
**作者**: C. L. Jackson et al.
**摘要**: 通过体外重组表达HEL308蛋白，分析其三维结构及ATP依赖性解旋酶活性。研究表明HEL308优先解链含有单链区域的DNA底物，并推测其功能与复制后修复及DNA损伤耐受有关。
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**说明**：HEL308(也称HELQ或DNA helicase HEL308)是DNA损伤修复和复制叉保护的关键蛋白。上述文献侧重于其重组蛋白的功能机制，涉及解旋酶活性、修复通路调控及结构研究。如需具体实验细节，建议结合全文进一步查阅。
**Background of Recombinant Human HEL308 Protein**
HEL308. a conserved DNA helicase, belongs to the Ski2-like helicase family and plays a critical role in genome maintenance. It is implicated in DNA repair, replication fork regression, and replication stress response, functioning primarily through its ATP-dependent 3’→5’ helicase activity. The human homolog, also known as HELQ, interacts with replication and repair machinery to stabilize or restart stalled replication forks, thereby safeguarding genome integrity.
Recombinant human HEL308 protein is typically produced using heterologous expression systems (e.g., *E. coli* or insect cells) for biochemical and cellular studies. Its study has advanced understanding of helicase mechanisms in homologous recombination (HR)-associated repair and Holliday junction processing. Dysregulation of HEL308 is linked to genomic instability and cancer predisposition, as mutations may impair DNA damage tolerance. Additionally, HEL308 interacts with BRCA complexes, suggesting roles in cancer biology and therapy resistance.
Research on recombinant HEL308 aids in elucidating helicase functions in replication stress survival, with potential applications in targeting DNA repair pathways for anticancer strategies. Its structural and functional analysis remains vital for exploring helicase-mediated genome stability mechanisms.
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