| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2BK |
| Uniprot No | O60814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-126aa |
| 氨基酸序列 | MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK |
| 分子量 | 39.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST1H2BK蛋白的参考文献示例，内容基于学术领域相关研究方向整理：
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1. **文献名称**：*"Expression and purification of recombinant human HIST1H2BK in Escherichia coli for chromatin assembly studies"*
**作者**：Happel N., Doenecke D.
**摘要**：研究描述了HIST1H2BK组蛋白在大肠杆菌中的重组表达及纯化方法，验证其用于体外核小体组装的功能，证明其与DNA结合的能力与传统组蛋白H2B相似。
2. **文献名称**：*"HIST1H2BK post-translational modifications in epigenetic regulation of pluripotent stem cells"*
**作者**：Zhang Y., et al.
**摘要**：探讨HIST1H2BK的磷酸化与乙酰化修饰对胚胎干细胞多能性维持的影响，通过重组蛋白突变实验揭示特定修饰位点在染色质开放性调控中的作用。
3. **文献名称**：*"Functional redundancy among HIST1H2B subtypes revealed by CRISPR/Cas9 and recombinant protein rescue assays"*
**作者**：Sato M., et al.
**摘要**：利用CRISPR敲除HIST1H2BK基因后，通过重组蛋白回补实验证明其与其他H2B亚型(如HIST1H2BJ)在DNA损伤修复中的功能冗余性。
4. **文献名称**：*"Recombinant HIST1H2BK facilitates nuclear reprogramming in somatic cell cloning"*
**作者**：Kim H., Gurdon J.B.
**摘要**：发现重组HIST1H2BK蛋白可提高体细胞核移植中染色体重塑效率，为改善克隆胚胎发育提供新策略。
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注：以上文献为示例性内容，实际研究中建议通过PubMed、Web of Science等平台检索最新文献。
Recombinant human HIST1H2BK protein is a genetically engineered form of the histone H2B type 1-K, a core component of nucleosomes in eukaryotic cells. As part of the HIST1 cluster on chromosome 6. HIST1H2BK encodes one of multiple H2B variants involved in chromatin structure and epigenetic regulation. Histones like H2B play essential roles in DNA packaging, gene expression modulation, and maintaining genome stability by organizing DNA into repeating nucleosomal units. The recombinant version is typically produced in bacterial or mammalian expression systems, enabling controlled studies of its interactions with DNA, histone partners (e.g., H2A, H3. H4), and post-translational modifications (e.g., phosphorylation, ubiquitination) that regulate processes like transcription and DNA repair. Researchers utilize recombinant HIST1H2BK to investigate chromatin remodeling mechanisms, epigenetic inheritance, and diseases linked to histone dysfunction, such as cancers or developmental disorders. Its purity and lack of endogenous modifications in experimental setups make it invaluable for biochemical assays, structural studies (e.g., X-ray crystallography), and reconstituting nucleosomes in vitro. Recent studies also explore its role in immune responses, as extracellular histones can act as damage-associated molecular patterns (DAMPs).
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