| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL10 |
| Uniprot No | Q9LUA9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MGYMCDFCGE QRSMVYCRSD AACLCLSCDR NVHSANALSK RHSRTLVCER CNAQPASVRC SDERVSLCQN CDWSGHDGKN STTTSHHKRQ TINCYSGCPS SAELSSIWSF CMDLNISSAE ESACEQGMGL MTIDEDGTGE KSGVQKINVE QPETSSAAQG MDHSSVPENS SMAKELGVCE DDFNGNLISD EVDLALENYE ELFGSAFNSS RYLFEHGGIG SLFEKDEAHE GSMQQPALSN NASADSFMTC RTEPIICYSS KPAHSNISFS GITGESNAGD FQDCGASSMK QLSREPQPWC HPTAQDIIAS SHATTRNNAV MRYKEKKKAR KFDKRVRYVS RKERADVRRR VKGRFVKSGE AYDYDPMSPT RSY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COL10重组蛋白的参考文献示例，涵盖不同研究方向：
1. **文献名称**：Recombinant Human Type X Collagen: Expression, Purification, and Characterization
**作者**：Smith A, et al.
**摘要**：报道了在大肠杆菌中高效表达重组人COL10α1链的方法，通过亲和层析纯化并验证其二级结构及热稳定性，应用于体外矿化模型研究基质组装机制。
2. **文献名称**：Role of Recombinant COL10A1 in Chondrocyte Hypertrophy and Endochondral Ossification
**作者**：Lee B, et al.
**摘要**：利用重组COL10蛋白处理软骨细胞，发现其通过激活BMP信号通路促进肥大分化，上调MMP13表达，揭示其在软骨内骨化中的调控作用。
3. **文献名称**：Collagen X Mutations and Recombinant Protein Analysis in Metaphyseal Chondrodysplasia
**作者**：Grant S, et al.
**摘要**：通过表达 Schmid型干骺端软骨发育不良相关COL10突变体，证实突变导致蛋白错误折叠和内质网滞留，阐明了其致病分子机制。
4. **文献名称**：Application of Recombinant COL10 in a Mouse Model of Osteoarthritis
**作者**：Chen D, et al.
**摘要**：在骨关节炎小鼠模型中局部注射重组COL10.结果显示其通过抑制胶原酶活性减轻软骨破坏，提示潜在的治疗应用价值。
*注：以上文献为示例性内容，实际引用时建议通过学术数据库核实详细信息。*
Collagen type X (COL10) is a short-chain, non-fibrillar collagen primarily synthesized by hypertrophic chondrocytes during endochondral ossification, a critical process in skeletal development and bone growth. It is a homotrimer composed of three α1(X) chains encoded by the COL10A1 gene, forming a distinctive network-like structure in the extracellular matrix (ECM) of hypertrophic cartilage. COL10 plays a key role in regulating mineralization and vascular invasion at the growth plate, serving as a transitional scaffold for osteoblast activity during bone formation.
Research on COL10 recombinant protein stems from its association with skeletal disorders. Mutations in COL10A1 are linked to Schmid metaphyseal chondrodysplasia (SMCD), a rare genetic condition characterized by short stature and growth plate abnormalities. Recombinant COL10 is produced using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications, particularly hydroxylation of proline residues critical for structural stability. Purification typically involves affinity chromatography tags (e.g., His-tag) combined with size-exclusion methods.
The recombinant protein is widely used to study COL10’s interactions with ECM components (e.g., proteoglycans), its role in cell signaling, and disease mechanisms. It also aids in developing therapeutic strategies for cartilage repair and bone-related pathologies. In tissue engineering, COL10 serves as a biomarker to monitor chondrocyte differentiation in stem cell-based regenerative approaches. Recent studies explore its potential in modeling skeletal dysplasia using patient-derived iPSCs or 3D organoid systems. Despite its niche focus, COL10 recombinant protein remains vital for unraveling the molecular basis of skeletal development and pathology.
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