| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AEBP1 |
| Uniprot No | Q8IUX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1158aa |
| 氨基酸序列 | MAAVRGAPLLSCLLALLALCPGGRPQTVLTDDEIEEFLEGFLSELEPEPREDDVEAPPPPEPTPRVRKAQAGGKPGKRPGTAAEVPPEKTKDKGKKGKKDKGPKVPKESLEGSPRPPKKGKEKPPKATKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKKPPSGKRPPILAPSETLEWPLPPPPSPGPEELPQEGGAPLSNNWQNPGEETHVEAREHQPEPEEETEQPTLDYNDQIEREDYEDFEYIRRQKQPRPPPSRRRRPERVWPEPPEEKAPAPAPEERIEPPVKPLLPPLPPDYGDGYVIPNYDDMDYYFGPPPPQKPDAERQTDEEKEELKKPKKEDSSPKEETDKWAVEKGKDHKEPRKGEELEEEWTPTEKVKCPPIGMESHRIEDNQIRASSMLRHGLGAQRGRLNMQTGATEDDYYDGAWCAEDDARTQWIEVDTRRTTRFTGVITQGRDSSIHDDFVTTFFVGFSNDSQTWVMYTNGYEEMTFHGNVDKDTPVLSELPEPVVARFIRIYPLTWNGSLCMRLEVLGCSVAPVYSYYAQNEVVATDDLDFRHHSYKDMRQLMKVVNEECPTITRTYSLGKSSRGLKIYAMEISDNPGEHELGEPEFRYTAGIHGNEVLGRELLLLLMQYLCREYRDGNPRVRSLVQDTRIHLVPSLNPDGYEVAAQMGSEFGNWALGLWTEEGFDIFEDFPDLNSVLWGAEERKWVPYRVPNNNLPIPERYLSPDATVSTEVRAIIAWMEKNPFVLGANLNGGERLVSYPYDMARTPTQEQLLAAAMAAARGEDEDEVSEAQETPDHAIFRWLAISFASAHLTLTEPYRGGCQAQDYTGGMGIVNGAKWNPRTGTINDFSYLHTNCLELSFYLGCDKFPHESELPREWENNKEALLTFMEQVHRGIKGVVTDEQGIPIANATISVSGINHGVKTASGGDYWRILNPGEYRVTAHAEGYTPSAKTCNVDYDIGATQCNFILARSNWKRIREIMAMNGNRPIPHIDPSRPMTPQQRRLQQRRLQHRLRLRAQMRLRRLNATTTLGPHTVPPTLPPAPATTLSTTIEPWGLIPPTTAGWEESETETYTEVVTEFGTEVEPEFGTKVEPEFETQLEPEFETQLEPEFEEEEEEEKEEEIATGQAFPFTTVETYTVNFGDF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AEBP1重组蛋白的3篇参考文献摘要示例(注：文献信息为虚拟示例，实际引用需核实原文)：
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1. **文献名称**: *AEBP1 promotes macrophage infiltration and extracellular matrix remodeling in glioblastoma*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组AEBP1蛋白体外处理胶质瘤细胞，发现其通过激活NF-κB通路增强肿瘤相关巨噬细胞的趋化迁移能力，并促进细胞外基质中胶原蛋白的异常沉积，加速肿瘤微环境重塑。
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2. **文献名称**: *Recombinant AEBP1 exacerbates vascular smooth muscle inflammation via TLR4-dependent signaling*
**作者**: Lee S, Kim JH.
**摘要**: 利用大肠杆菌表达系统纯化的重组AEBP1蛋白，在动脉粥样硬化模型中被证实可直接结合血管平滑肌细胞表面TLR4受体，诱导促炎因子(如IL-6、TNF-α)分泌，加剧血管壁炎症反应。
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3. **文献名称**: *In vitro characterization of AEBP1 recombinant protein in adipocyte differentiation*
**作者**: Wang X, et al.
**摘要**: 通过哺乳动物细胞表达体系获得功能性AEBP1重组蛋白，实验表明其通过抑制PPARγ转录活性阻碍脂肪细胞分化，揭示其在肥胖相关代谢调控中的潜在作用机制。
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如需实际文献，建议在PubMed等数据库以关键词“AEBP1 recombinant protein”或“AEBP1 expression and function”检索近年研究。
**Background of AEBP1 Recombinant Protein**
AEBP1 (Adipocyte Enhancer-Binding Protein 1), also known as aortic carboxypeptidase-like protein (ACLP), is a multifunctional protein encoded by the *AEBP1* gene. It belongs to the carboxypeptidase family but lacks enzymatic activity due to mutations in its catalytic domain. Structurally, AEBP1 contains an N-terminal signal peptide, a collagen-binding domain, a thrombospondin type 1 repeat (TSR), and a C-terminal discoidin-like domain. It exists in two isoforms: a secreted form that interacts with the extracellular matrix (ECM) and a nuclear form implicated in transcriptional regulation.
AEBP1 plays critical roles in adipogenesis, inflammation, and tissue remodeling. Initially identified as a transcriptional repressor in adipocyte differentiation, it suppresses PPARγ, a master regulator of adipogenesis. In the ECM, secreted AEBP1 modulates collagen fibrillogenesis and interacts with integrins to influence cell adhesion and migration. Dysregulation of AEBP1 is linked to pathological conditions, including atherosclerosis, obesity, and cancer. Overexpression of AEBP1 is observed in glioblastoma, breast cancer, and other malignancies, where it promotes tumor progression, invasion, and chemoresistance by activating pathways like NF-κB and PI3K/Akt.
Recombinant AEBP1 protein is produced using expression systems such as *E. coli* or mammalian cells, enabling studies of its biochemical and functional properties. Purification typically involves affinity tags (e.g., His-tag) and chromatography. Researchers utilize recombinant AEBP1 to investigate its roles in ECM organization, cellular signaling, and disease mechanisms, as well as to screen therapeutic inhibitors targeting its oncogenic or pro-fibrotic activities. Its dual localization (secreted/nuclear) and diverse interactions make AEBP1 a compelling subject for understanding tissue homeostasis and disease pathogenesis.
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