| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL16 |
| Uniprot No | Q8RWD0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417aa |
| 氨基酸序列 | MMKSLANAVG AKTARACDSC VKRRARWYCA ADDAFLCQSC DSLVHSANPL ARRHERVRLK TASPAVVKHS NHSSASPPHE VATWHHGFTR KARTPRGSGK KNNSSIFHDL VPDISIEDQT DNYELEEQLI CQVPVLDPLV SEQFLNDVVE PKIEFPMIRS GLMIEEEEDN AESCLNGFFP TDMELEEFAA DVETLLGRGL DTESYAMEEL GLSNSEMFKI EKDEIEEEVE EIKAMSMDIF DDDRKDVDGT VPFELSFDYE SSHKTSEEEV MKNVESSGEC VVKVKEEEHK NVLMLRLNYD SVISTWGGQG PPWSSGEPPE RDMDISGWPA FSMVENGGES THQKQYVGGC LPSSGFGDGG REARVSRYRE KRRTRLFSKK IRYEVRKLNA EKRPRMKGRF VKRASLAAAA SPLGVNY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COL16重组蛋白的3篇参考文献示例(注：文献信息为模拟内容，实际引用需核实)：
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1. **文献名称**: *"Recombinant human collagen XVI regulates keratinocyte adhesion and migration in vitro"*
**作者**: Kassner A, et al.
**摘要**: 研究利用重组COL16蛋白探讨其对表皮角质形成细胞粘附、迁移的影响，发现COL16通过整合素α1β1调控细胞外基质相互作用，提示其在皮肤修复中的作用。
2. **文献名称**: *"Expression and functional characterization of recombinant collagen XVI in fibrosis models"*
**作者**: Gröschl D, et al.
**摘要**: 通过哺乳动物细胞表达系统制备重组COL16蛋白，证实其与TGF-β信号通路协同促进成纤维细胞活化，可能参与纤维化疾病的病理过程。
3. **文献名称**: *"Collagen XVI interacts with integrins to reshape tumor microenvironment"*
**作者**: Räth M, et al.
**摘要**: 研究重组COL16蛋白在肿瘤基质中的功能，发现其通过结合整合素α2β1增强癌细胞侵袭能力，为靶向肿瘤微环境提供潜在策略。
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建议通过PubMed或Web of Science以关键词“COL16A1 recombinant”“collagen XVI expression”检索最新文献，并核实具体研究内容。
**Background of COL16 (Collagen XVI) Recombinant Protein**
Collagen XVI (COL16) is a member of the fibril-associated collagens with interrupted triple helices (FACIT) family, encoded by the *COL16A1* gene. It is a non-fibrillar collagen predominantly found in basement membrane zones (BMZs) of tissues such as skin, blood vessels, and muscle, where it interacts with other extracellular matrix (ECM) components like collagen I, IV, and integrins. Structurally, COL16 consists of multiple collagenous domains interrupted by non-collagenous regions, enabling flexible interactions within the ECM.
Recombinant COL16 protein is engineered to study its biological roles, including ECM organization, cell adhesion, and tissue homeostasis. Its production typically involves mammalian or insect cell systems to ensure proper post-translational modifications, such as hydroxylation of proline residues critical for collagen stability. Purification strategies often employ affinity tags (e.g., His-tag) combined with chromatographic techniques.
Research highlights COL16's involvement in modulating cell-matrix signaling, particularly in skin fibrosis, wound healing, and vascular remodeling. It also plays a role in cancer progression by influencing tumor-stroma interactions. Dysregulation of COL16 is linked to connective tissue disorders and fibrotic diseases.
Despite its emerging significance, COL16's functional mechanisms remain less explored compared to other collagens. Recombinant COL16 serves as a vital tool to dissect its structure-function relationships, therapeutic potential, and diagnostic applications in ECM-related pathologies. Current studies focus on its utility in tissue engineering and regenerative medicine, aiming to harness its ECM-modulating properties for clinical interventions.
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