| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRMT1L4 |
| Uniprot No | Q9NR22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 61-394aa |
| 氨基酸序列 | MSKLLNPEEMTSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGIECSSISDYSEKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR |
| 分子量 | 45.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人HRMT1L4蛋白的研究文献目前公开记录较少，可能存在名称拼写误差(例如是否为PRMT家族相关蛋白?)。以下是基于主题构造的示例性文献，供参考：
1. **文献名称**：*Cloning and Functional Analysis of Recombinant Human HRMT1L4 Protein*
**作者**：Chen et al. (2018)
**摘要**：描述了HRMT1L4蛋白在大肠杆菌系统中的重组表达与纯化方法，并验证其甲基转移酶活性，揭示其在组蛋白修饰中的潜在作用。
2. **文献名称**：*HRMT1L4-mediated Methylation Regulates Tumor Suppressor Pathways*
**作者**：Zhang et al. (2020)
**摘要**：通过体外重组蛋白实验，证明HRMT1L4通过甲基化p53蛋白的特定精氨酸位点，影响其在结直肠癌中的稳定性与功能。
3. **文献名称**：*Structural Characterization of Human HRMT1L4 by Cryo-EM*
**作者**：Wang et al. (2021)
**摘要**：利用冷冻电镜解析了重组HRMT1L4蛋白的三维结构，揭示了其底物结合口袋及催化机制，为药物设计提供依据。
**建议**：
- 核实蛋白名称准确性(如是否属于PRMT家族或存在不同命名变体)。
- 通过PubMed或UniProt数据库(检索号如Q8N2K1)查询最新研究。
**Background of Recombinant Human HRMT1L4 Protein**
HRMT1L4 (Human Histone-Related Methyltransferase 1-Like 4), also known as METTL4 in some contexts, is a member of the methyltransferase enzyme family. This protein plays a role in post-translational modifications, specifically catalyzing the transfer of methyl groups to target biomolecules, such as nucleic acids or proteins, influencing their activity, stability, or interaction partners. HRMT1L4 is implicated in epigenetic regulation, potentially modifying histones or RNA species to impact gene expression, chromatin structure, or cellular differentiation.
Recombinant HRMT1L4 is engineered in vitro using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional protein for research. Its recombinant form enables studies on substrate specificity, enzyme kinetics, and its role in pathways linked to cancer, development, or stress responses. Structural studies often focus on its conserved catalytic domains, such as the SAM (S-adenosylmethionine)-binding motif essential for methyltransferase activity. Current research aims to clarify its physiological targets and explore therapeutic potential in diseases involving epigenetic dysregulation. Despite its emerging significance, mechanistic details and broader biological networks remain under investigation.
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