| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTATIP |
| Uniprot No | Q92993 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MAEVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW |
| 分子量 | 0 |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HTATIP蛋白的3篇代表性文献及其摘要概括(文献信息为示例性描述)：
1. **"HIV-1 Tat-interactive protein HTATIP inhibits cancer cell proliferation and induces apoptosis"**
*作者：Zhang, Q. et al.*
**摘要**：本研究通过原核表达系统成功制备了重组人HTATIP蛋白，发现其可通过调控组蛋白乙酰化抑制肿瘤细胞增殖并激活caspase依赖性凋亡通路，提示其在癌症治疗中的潜在应用。
2. **"Structural analysis of recombinant human HTATIP reveals its role as a histone acetyltransferase inhibitor"**
*作者：Chen, L. & Wang, H.*
**摘要**：利用X射线晶体学解析了重组HTATIP蛋白的三维结构，发现其C端结构域能与组蛋白乙酰转移酶结合并抑制酶活性，为阐明其在表观遗传调控中的机制提供结构基础。
3. **"HTATIP reprograms glucose metabolism in hepatocellular carcinoma via disrupting HIF-1α/p300 complex"**
*作者：Kim, S. et al.*
**摘要**：通过重组HTATIP蛋白处理肝癌细胞，证实其可通过竞争性结合p300蛋白破坏HIF-1α复合体，抑制糖酵解关键基因表达，逆转肿瘤代谢重编程现象。
注：以上内容基于HTATIP已知功能(如组蛋白修饰、凋亡调控和肿瘤抑制)的典型研究方向模拟生成，实际文献需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar以“recombinant HTATIP protein”等关键词检索获取。建议结合具体研究方向补充近年高影响因子论文。
Recombinant human HTATIP protein (also known as TIP60 or KAT5) is a crucial histone acetyltransferase involved in chromatin remodeling, transcriptional regulation, and DNA repair. Initially identified as an HIV-1 Tat-interacting protein, HTATIP plays dual roles in viral replication and host cell processes. It acetylates histones (e.g., H3. H4) and non-histone targets (e.g., p53. ATM kinase), modulating gene expression, DNA damage response, apoptosis, and cell cycle progression. In HIV infection, HTATIP interacts with the viral Tat protein to enhance viral transcription while promoting apoptosis of infected cells. Recombinant HTATIP is produced in bacterial or mammalian expression systems, enabling functional studies of its enzymatic activity, protein interactions, and structural features. Its applications span mechanistic research on epigenetic regulation, cancer biology (as both tumor suppressor and oncogene), viral pathogenesis, and drug discovery targeting acetylation pathways. Variants or mutations in HTATIP are linked to cancers and neurodegenerative disorders, underscoring its biomedical relevance. The protein's N-terminal chromodomain and C-terminal acetyltransferase domain are key to its substrate recognition and catalytic functions.
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