| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREBBP |
| Uniprot No | Q92793 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1319-1710aa |
| 氨基酸序列 | RK ENKFSAKRLQ TTRLGNHLED RVNKFLRRQN HPEAGEVFVR VVASSDKTVE VKPGMKSRFV DSGEMSESFP YRTKALFAFE EIDGVDVCFF GMHVQEYGSD CPPPNTRRVY ISYLDSIHFF RPRCLRTAVY HEILIGYLEY VKKLGYVTGH IWACPPSEGD DYIFHCHPPD QKIPKPKRLQ EWYKKMLDKA FAERIIHDYK DIFKQATEDR LTSAKELPYF EGDFWPNVLE ESIKELEQEE EERKKEESTA ASETTEGSQG DSKNAKKKNN KKTNKNKSSI SRANKKKPSM PNVSNDLSQK LYATMEKHKE VFFVIHLHAG PVINTLPPIV DPDPLLSCDL MDGRDAFLTL ARDKHWEFSS LRRSKWSTLC MLVELHTQGQ DRFVYTCNEC |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于CREBBP重组蛋白的参考文献及其简要摘要：
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1. **"Structural analysis of the CREBBP acetyltransferase domain in complex with a synthetic coactivator"**
*Authors: Roelfsema et al.*
**摘要**：通过X射线晶体学解析了CREBBP乙酰转移酶结构域与人工合成的共激活因子复合物的三维结构，揭示了其底物识别和催化机制，为靶向CREBBP的药物设计提供了结构基础。
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2. **"CREBBP/p300 interacts with STAT1 and enhances its DNA-binding activity"**
*Authors: Horvai et al.*
**摘要**：研究发现重组CREBBP/p300蛋白通过直接结合STAT1增强其与DNA的亲和力，证明了CREBBP在干扰素信号通路中的调控作用，并揭示了其参与免疫应答的分子机制。
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3. **"CREBBP mutations in hematopoietic malignancies disrupt histone acetylation and transcriptional regulation"**
*Authors: Pasqualucci et al.*
**摘要**：分析了白血病和淋巴瘤患者中CREBBP基因突变对重组蛋白功能的影响，发现突变导致组蛋白乙酰化活性降低，破坏关键基因(如BCL6)的转录抑制，促进肿瘤发生。
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4. **"Recombinant CREBBP protein delivery rescues cognitive deficits in a mouse model of Rubinstein-Taybi syndrome"**
*Authors: Liu et al.*
**摘要**：利用重组CREBBP蛋白通过病毒载体递送至Rubinstein-Taybi综合征模型小鼠脑内，发现其可恢复组蛋白乙酰化水平并改善学习记忆缺陷，为基因治疗提供了实验依据。
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以上文献涵盖CREBBP重组蛋白的结构、功能、疾病关联及治疗应用方向，可根据具体研究方向进一步筛选。
CREBBP (cAMP-response element-binding protein-binding protein), also known as CBP or KAT3A, is a ubiquitously expressed transcriptional coactivator that plays a critical role in regulating gene expression through chromatin remodeling and interaction with transcription factors. The full-length CREBBP protein contains multiple functional domains, including a histone acetyltransferase (HAT) domain, a KIX domain for binding phosphorylated transcription factors, and a bromodomain for acetyl-lysine recognition. Recombinant CREBBP proteins are engineered versions typically produced in bacterial or eukaryotic expression systems, retaining key functional regions to study molecular interactions or enzymatic activity.
As a central node in cellular signaling, CREBBP integrates inputs from pathways such as cAMP, MAPK, and TGF-β by acetylating histones (enhancing chromatin accessibility) and serving as a scaffold for transcriptional complexes. Its HAT activity mediates acetylation of histone H3 lysine 18 (H3K18ac), a hallmark of active enhancers. Recombinant CREBBP fragments are widely used to investigate mechanisms of transcriptional regulation, epigenetic inheritance, and protein-protein interactions in vitro.
Mutations in CREBBP are associated with Rubinstein-Taybi syndrome (characterized by developmental abnormalities) and various cancers. Recombinant proteins enable functional studies of disease-associated variants, particularly in hematological malignancies where CREBBP loss disrupts differentiation. Therapeutic strategies targeting CREBBP/EP300 complexes in oncology have driven demand for purified recombinant proteins for drug screening and structural studies. Current production methods often incorporate affinity tags (e.g., GST, His-tag) and optimize conditions to preserve HAT activity. Quality control typically includes SDS-PAGE verification and enzymatic activity assays using histone substrates. These tools continue to advance research in epigenetics and targeted cancer therapies.
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