| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTMR12 |
| Uniprot No | Q9C0I1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747 aa |
| 活性数据 | MLGKGVVGGG GGTKAPKPSF VSYVRPEEIH TNEKEVTEKE VTLHLLPGEQ LLCEASTVLK YVQEDSCQHG VYGRLVCTDF KIAFLGDDES ALDNDETQFK NKVIGENDIT LHCVDQIYGV FDEKKKTLFG QLKKYPEKLI IHCKDLRVFQ FCLRYTKEEE VKRIVSGIIH HTQAPKLLKR LFLFSYATAA QNNTVTDPKN HTVMFDTLKD WCWELERTKG NMKYKAVSVN EGYKVCERLP AYFVVPTPLP EENVQRFQGH GIPIWCWSCH NGSALLKMSA LPKEQDDGIL QIQKSFLDGI YKTIHRPPYE IVKTEDLSSN FLSLQEIQTA YSKFKQLFLI DNSTEFWDTD IKWFSLLESS SWLDIIRRCL KKAIEITECM EAQNMNVLLL EENASDLCCL ISSLVQLMMD PHCRTRIGFQ SLIQKEWVMG GHCFLDRCNH LRQNDKEEVP VFLLFLDCVW QLVHQHPPAF EFTETYLTVL SDSLYIPIFS TFFFNSPHQK DTNMGREGQD TQSKPLNLLT VWDWSVQFEP KAQTLLKNPL YVEKPKLDKG QRKGMRFKHQ RQLSLPLTQS KSSPKRGFFR EETDHLIKNL LGKRISKLIN SSDELQDNFR EFYDSWHSKS TDYHGLLLPH IEGPEIKVWA QRYLRWIPEA QILGGGQVAT LSKLLEMMEE VQSLQEKIDE RHHSQQAPQA EAPCLLRNSA RLSSLFPFAL LQRHSSKPVL PTSGWKALGD EDDLAKREDE FVDLGDV |
| 分子量 | 86.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTMR12蛋白的3篇参考文献(因研究领域较窄，文献有限，以下为模拟示例)：
1. **文献名称**：*MTMR12 Regulates Autophagy via Interaction with Beclin 1*
**作者**：Li, X. et al.
**摘要**：研究发现MTMR12通过与自噬关键蛋白Beclin 1相互作用，调控自噬体的形成。重组人MTMR12蛋白实验表明其磷酸酶活性对自噬通路的负向调控至关重要。
2. **文献名称**：*Structural and Functional Analysis of MTMR12 Phosphatase Activity*
**作者**：Smith, J.R. & Wang, Y.
**摘要**：通过重组表达MTMR12蛋白并解析其晶体结构，揭示了其催化底物特异性及与其他肌管素相关蛋白的差异，提示其在磷酸肌醇代谢中的独特作用。
3. **文献名称**：*MTMR12 Modulates Insulin Signaling through PI3K/AKT Pathway*
**作者**：Chen, L. et al.
**摘要**：研究利用重组MTMR12蛋白发现其可结合磷脂酰肌醇3-磷酸(PI3P)，负调控胰岛素信号通路，为代谢疾病相关机制提供了新视角。
**说明**：MTMR12相关研究相对较少，实际文献可能需通过专业数据库检索。上述示例基于该蛋白已知功能(如磷酸酶活性、自噬调控)的合理推测，建议使用PubMed/Google Scholar以“MTMR12”或“myotubularin-related protein 12”为关键词查找最新文献。
Myotubularin-related protein 12 (MTMR12), also known as 3-PAP, is a member of the myotubularin phosphatase family, which primarily regulates intracellular signaling through hydrolysis of phosphoinositide lipids. Unlike catalytically active myotubularins, MTMR12 belongs to the "pseudophosphatase" subgroup due to amino acid substitutions in its active site, rendering it enzymatically inactive. It is widely expressed in human tissues and localizes to cytoplasmic vesicles, endosomes, and the plasma membrane.
MTMR12 functions as an adaptor protein, forming heterodimers with active phosphatase counterparts like MTMR2 or MTMR3. These interactions enhance the stability, subcellular targeting, and lipid substrate specificity of its partners, thereby modulating phosphoinositide metabolism. This regulatory role is critical for membrane trafficking, autophagy, and endocytic processes. Recent studies suggest MTMR12 involvement in neuronal development and muscle cell differentiation. Its dysregulation has been linked to neurodevelopmental disorders and certain cancers, though mechanistic insights remain limited.
As a recombinant protein, MTMR12 is utilized in structural studies to map interaction domains and in high-throughput screens to identify small molecule modulators of phosphoinositide signaling pathways. Ongoing research aims to clarify its physiological relevance in disease contexts and explore therapeutic potential.
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