| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTHFD1L |
| Uniprot No | Q6UB35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-366 aa |
| 活性数据 | MAVLALTDSLADMKARLGRMVVASDKSGQPVTADDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF |
| 分子量 | 65.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTHFD1L蛋白的3篇代表性文献的简化信息(注意：文献信息为示例，可能需通过数据库核对准确性)：
1. **文献名称**：*Mitochondrial one-carbon metabolism in folate targeting*
**作者**：Tibbetts, A.S., Appling, D.R.
**摘要**：早期研究线粒体叶酸代谢的关键酶MTHFD1L的功能，探讨其通过催化一碳单位(如甲酸盐)的代谢，支持核苷酸生物合成的机制。
2. **文献名称**：*Structure and function of the human mitochondrial MTHFD1L*
**作者**：Pike, S.T., et al.
**摘要**：解析了重组人MTHFD1L蛋白的酶活性，表明其通过脱氢酶和环水解酶反应参与线粒体内甲酸盐生成，影响一碳代谢通路。
3. **文献名称**：*MTHFD1L variants and neural tube defects*
**作者**：Christensen, K.E., et al.
**摘要**：研究了MTHFD1L基因多态性与神经管缺陷的关联，发现其蛋白功能异常可能导致叶酸代谢紊乱，影响胚胎发育。
**提示**：
- 建议通过PubMed或Google Scholar输入关键词 **"MTHFD1L recombinant"、"MTHFD1L enzyme function"** 获取最新文献。
- 重点可关注癌症或先天性疾病领域的相关研究，近年可能涉及该蛋白的调控机制或治疗潜力。
MTHFD1L (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like) is a mitochondrial enzyme critical in one-carbon metabolism, a biochemical network essential for nucleotide synthesis, methylation reactions, and cellular redox balance. This bifunctional protein catalyzes the NADP+-dependent dehydrogenase and cyclohydrolase reactions, converting 10-formyltetrahydrofolate to 5.10-methenyltetrahydrofolate and subsequently to 5-formyltetrahydrofolate. By regulating formate production, MTHFD1L bridges mitochondrial and cytosolic one-carbon pools, influencing purine and thymidine biosynthesis. Its activity is particularly vital in rapidly dividing cells, such as cancer cells, which rely heavily on one-carbon units for proliferation. Dysregulation of MTHFD1L has been implicated in neural tube defects and cancer progression. Recombinant human MTHFD1L protein, typically expressed in Escherichia coli or mammalian systems, enables mechanistic studies of mitochondrial folate metabolism, enzyme kinetics, and inhibitor screening for therapeutic development. Structural studies using recombinant protein have revealed insights into its substrate-binding domains and regulatory mechanisms, aiding drug design targeting one-carbon metabolism disorders.
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