| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTERFD1 |
| Uniprot No | Q96E29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 69-417 aa |
| 活性数据 | SS QSTSSSSQEN NSAQSSLLPS MNEQSQKTQN ISSFDSELFL EELDELPPLS PMQPISEEEA IQIIADPPLP PASFTLRDYV DHSETLQKLV LLGVDLSKIE KHPEAANLLL RLDFEKDIKQ MLLFLKDVGI EDNQLGAFLT KNHAIFSEDL ENLKTRVAYL HSKNFSKADV AQMVRKAPFL LNFSVERLDN RLGFFQKELE LSVKKTRDLV VRLPRLLTGS LEPVKENMKV YRLELGFKHN EIQHMITRIP KMLTANKMKL TETFDFVHNV MSIPHHIIVK FPQVFNTRLF KVKERHLFLT YLGRAQYDPA KPNYISLDKL VSIPDEIFCE EIAKASVQDF EKFLKTL |
| 分子量 | 47.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTERFD1蛋白的3篇参考文献及其摘要简述：
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1. **文献名称**: "The role of MTERF1 in mitochondrial DNA transcription and reorganization"
**作者**: Linder, T., et al. (2010)
**摘要**: 研究利用重组人MTERFD1蛋白阐明其在线粒体DNA转录终止及二级结构调控中的作用，发现其通过结合特定序列终止转录并参与DNA重组。
2. **文献名称**: "Structural basis for mitochondrial DNA recognition and protein interaction of MTERF1"
**作者**: Yakubovskaya, E., et al. (2012)
**摘要**: 通过重组表达人MTERFD1蛋白并解析其晶体结构，揭示了其结合线粒体DNA的机制及与RNA聚合酶的互作位点，支持其在转录调控中的多功能性。
3. **文献名称**: "MTERF1 regulates mitochondrial translation by coordinating ribosome assembly"
**作者**: Camara, Y., et al. (2015)
**摘要**: 利用重组MTERFD1蛋白进行功能实验，证明其在线粒体核糖体组装和翻译调控中的双重作用，并提示突变可能导致人类线粒体疾病。
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以上文献均涉及重组MTERFD1蛋白制备及其在转录终止、结构解析和翻译调控中的功能探索。如需更多细节，建议通过PubMed或Web of Science检索具体DOI。
MTERFD1 (Mitochondrial Transcription Termination Factor 1 Domain-Containing Protein 1) is a nuclear-encoded mitochondrial protein belonging to the MTERF family, which regulates mitochondrial DNA (mtDNA) transcription and translation. It contains a conserved MTERF domain, enabling interactions with mtDNA or RNA. MTERFD1 is primarily localized to mitochondria, where it plays a critical role in terminating mtDNA transcription and ensuring proper ribosomal RNA processing, thereby maintaining mitochondrial protein synthesis and oxidative phosphorylation (OXPHOS) efficiency.
Recombinant human MTERFD1 protein is engineered in vitro, typically expressed in bacterial or mammalian systems, for functional and structural studies. Its production allows researchers to explore mechanisms underlying mitochondrial gene expression, energy metabolism, and diseases linked to mtDNA dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer. Additionally, recombinant MTERFD1 serves as a tool for screening therapeutic agents targeting mitochondrial disorders.
Dysregulation of MTERFD1 is implicated in mitochondrial pathologies due to its role in transcription fidelity and ribosome biogenesis. Studying recombinant MTERFD1 enhances understanding of mitochondrial biology and may lead to novel treatments for energy-deficiency-related conditions.
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