| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTERFD2 |
| Uniprot No | Q7Z6M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-381 aa |
| 活性数据 | LTTASNGG VIEELSCVRS NNYVQEPECR RNLVQCLLEK QGTPVVQGSL ELERVMSSLL DMGFSNAHIN ELLSVRRGAS LQQLLDIISE FILLGLNPEP VCVVLKKSPQ LLKLPIMQMR KRSSYLQKLG LGEGKLKRVL YCCPEIFTMR QQDINDTVRL LKEKCLFTVQ QVTKILHSCP SVLREDLGQL EYKFQYAYFR MGIKHPDIVK SEYLQYSLTK IKQRHIYLER LGRYQTPDKK GQTQIPNPLL KDILRVSEAE FLARTACTSV EEFQVFKKLL AREEEESESS TSDDKRASLD EDEDDDDEED NDEDDNDEDD DDEDDDEAED NDEDEDDDEE E |
| 分子量 | 43.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开的文献数据库中关于MTERFD2蛋白的研究较为有限(截至2023年10月)，以下为基于相关领域知识的假设性参考文献示例，实际文献需通过PubMed、Google Scholar等平台验证：
1. **标题**: "MTERFD2 regulates mitochondrial transcription termination and genome stability"
**作者**: Chen L, et al.
**摘要**: 研究揭示了MTERFD2作为线粒体转录终止因子家族成员，通过重组蛋白实验证实其结合mtDNA特定区域，调控转录终止，并与线粒体基因组稳定性相关。
2. **标题**: "Structural insights into MTERFD2’s role in oxidative phosphorylation complex assembly"
**作者**: Garcia-Ruiz I, et al.
**摘要**: 通过重组人MTERFD2蛋白的晶体结构解析，发现其与线粒体核糖体蛋白相互作用，可能影响氧化磷酸化复合物的组装效率。
3. **标题**: "MTERFD2 knockout leads to impaired cellular energy metabolism via mitochondrial dysfunction"
**作者**: Tanaka K, et al.
**摘要**: 利用CRISPR技术构建MTERFD2敲除细胞系，发现其缺失导致线粒体呼吸链活性下降，重组蛋白回补实验可部分恢复代谢缺陷。
4. **标题**: "Interactome analysis of MTERFD2 reveals its association with neurodegenerative pathways"
**作者**: Müller S, et al.
**摘要**: 通过重组MTERFD2蛋白的免疫共沉淀实验，鉴定出与帕金森病相关蛋白的相互作用，提示其可能参与神经退行性疾病的线粒体病理机制。
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**提示**：建议使用以下关键词在学术平台检索最新进展：
`"MTERFD2" + mitochondrial + recombinant protein`
`"MTERFD2" + structure/function/gene expression`
MTERFD2 (Mitochondrial Transcription Termination Factor 2 Domain-containing protein 2) is a nuclear-encoded mitochondrial protein involved in regulating mitochondrial gene expression. It belongs to the MTERF family, known for roles in transcription, translation, and RNA metabolism within mitochondria. Human MTERFD2 contains a conserved MTERF4-like domain, suggesting potential interactions with mitochondrial DNA (mtDNA) or ribosomal components.
Though its precise molecular function remains under investigation, MTERFD2 is proposed to participate in mitochondrial ribosome biogenesis or transcription termination. Studies indicate it localizes to the mitochondrial matrix and interacts with mitochondrial ribosomal proteins, implicating it in coordinating translation machinery assembly. Dysregulation of mitochondrial gene expression, a process MTERFD2 may modulate, is linked to metabolic disorders, neurodegeneration, and cancer. Recombinant human MTERFD2 protein, typically produced via bacterial or mammalian expression systems with affinity tags (e.g., His-tag), serves as a vital tool for structural studies, interaction assays, and functional analyses. Research leveraging recombinant MTERFD2 aims to clarify its role in mitochondrial homeostasis, offering insights into diseases associated with mitochondrial dysfunction and potential therapeutic strategies. Further characterization of its binding partners and regulatory mechanisms could reveal novel targets for addressing energy metabolism-related pathologies.
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