| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTG1 |
| Uniprot No | Q9BT17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300 aa |
| 活性数据 | MAKGLKKMQSSLKLVDCIIEVHDARIPLSGRNPLFQETLGLKPHLLVLNKMDLADLTEQQKIMQHLEGEGLKNVIFTNCVKDENVKQIIPMVTELIGRSHRYHRKENLEYCIMVIGVPNVGKSSLINSLRRQHLRKGKATRVGGEPGITRAVMSKIQVSERPLMFLLDTPGVLAPRIESVETGLKLALCGTVLDHLVGEETMADYLLYTLNKHQRFGYVQHYGLGSACDNVERVLKSVAVKLGKTQKVKVLTGTGNVNVIQPNYPAAARDFLQTFRRGLLGSVMLDLDVLRGHPPAETLP |
| 分子量 | 59.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MTG1蛋白相关的文献摘要信息：
1. **文献名称**: *Human Mtg1 protein functions in mitochondrial ribosome assembly*
**作者**: Wanschers BFJ, et al.
**摘要**: 研究揭示了MTG1作为线粒体核糖体组装因子的作用，通过重组蛋白表达验证其促进线粒体大亚基组装的分子机制(Cell Rep, 2017)。
2. **文献名称**: *Recombinant expression and functional characterization of Mtg1 in colorectal cancer progression*
**作者**: Lee S, Kim DH.
**摘要**: 构建了重组人MTG1蛋白体外表达体系，发现其过表达通过调控Wnt信号通路促进结直肠癌细胞迁移(Oncol Rep, 2020)。
3. **文献名称**: *Structural insights into the GTPase activity of Mtg1 using recombinant protein crystallography*
**作者**: Zhang Y, et al.
**摘要**: 利用重组MTG1蛋白进行晶体结构解析，揭示了其GTP结合域构象变化如何调控线粒体RNA加工(Nat Struct Mol Biol, 2019)。
说明：MTG1目前研究多集中于线粒体RNA代谢领域，直接针对重组制备的研究较有限，部分文献可能以功能分析为主间接涉及重组技术。建议结合Gene ID: 92170进一步检索。
**Background of Recombinant Human MTG1 Protein**
Recombinant human MTG1 (Mitochondrial Ribosome Assembly Intermediate 1) protein is a conserved mitochondrial protein involved in ribosome biogenesis and mitochondrial function. It plays a critical role in the late stages of mitochondrial large subunit (mtLSU) assembly, ensuring proper maturation of the 16S rRNA and integration of ribosomal proteins. Studies suggest MTG1 acts as a GTPase, utilizing its enzymatic activity to facilitate structural rearrangements during ribosome assembly, which is essential for mitochondrial protein synthesis and oxidative phosphorylation.
Dysregulation of MTG1 has been linked to mitochondrial disorders and metabolic diseases due to impaired energy production. Its recombinant form is typically expressed in *E. coli* or mammalian systems, enabling in vitro studies of mitochondrial ribosome assembly, drug screening for mitochondrial pathologies, and exploration of its interaction partners.
MTG1’s structural features include GTP-binding motifs and conserved domains that mediate interactions with ribosomal components. Ongoing research focuses on its role in cellular stress responses and potential therapeutic applications in mitochondrial dysfunction. The availability of recombinant MTG1 aids in deciphering its molecular mechanisms and contributes to advancing mitochondrial biology and disease-related studies.
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