| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYBL1 |
| Uniprot No | P10243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-752 aa |
| 活性数据 | MAKRSRSEDEDDDLQYADHDYEVPQQKGLKKLWNRVKWTRDEDDKLKKLVEQHGTDDWTLIASHLQNRSDFQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSLIAKHLKGRIGKQCRERWHNHLNPEVKKSSWTEEEDRIIYEAHKRLGNRWAEIAKLLPGRTDNSIKNHWNSTMRRKVEQEGYLQDGIKSERSSSKLQHKPCAAMDHMQTQNQFYIPVQIPGYQYVSPEGNCIEHVQPTSAFIQQPFIDEDPDKEKKIKELEMLLMSAENEVRRKRIPSQPGSFSSWSGSFLMDDNMSNTLNSLDEHTSEFYSMDENQPVSAQQNSPTKFLAVEANAVLSSLQTIPEFAETLELIESDPVAWSDVTSFDISDAAASPIKSTPVKLMRIQHNEGAMECQFNVSLVLEGKKNTCNGGNSEAVPLTSPNIAKFSTPPAILRKKRKMRVGHSPGSELRDGSLNDGGNMALKHTPLKTLPFSPSQFFNTCPGNEQLNIENPSFTSTPICGQKALITTPLHKETTPKDQKENVGFRTPTIRRSILGTTPRTPTPFKNALAAQEKKYGPLKIVSQPLAFLEEDIREVLKEETGTDLFLKEEDEPAYKSCKQENTASGKKVRKSLVLDNWEKEESGTQLLTEDISDMQSENRFTTSLLMIPLLEIHDNRCNLIPEKQDINSTNKTYTLTKKKPNPNTSKVVKLEKNLQSNCEWETVVYGKTEDQLIMTEQARRYLSTYTATSSTSRALIL |
| 分子量 | 93.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人MYBL1蛋白相关的3篇文献示例(虚构示例，仅用于参考格式)：
1. **文献名称**：*"Functional Characterization of Recombinant Human MYBL1 in Transcriptional Regulation"*
**作者**：Smith J, et al.
**摘要**：研究通过体外表达重组人MYBL1蛋白，发现其通过结合特定DNA启动子区域激活下游基因(如细胞周期相关基因)，并揭示了其磷酸化修饰对转录活性的调控机制。
2. **文献名称**：*"MYBL1 Protein Structure and DNA-Binding Properties Revealed by X-ray Crystallography"*
**作者**：Chen L, Wang H.
**摘要**：解析了重组人MYBL1蛋白的晶体结构，阐明其MYB结构域与DNA结合的分子机制，为靶向MYBL1的抑制剂设计提供结构基础。
3. **文献名称**：*"Role of Recombinant MYBL1 in Glioblastoma Cell Proliferation"*
**作者**：Kim S, et al.
**摘要**：通过过表达重组MYBL1蛋白，证实其在胶质母细胞瘤中促进肿瘤细胞增殖和迁移，并通过RNA测序发现其调控的致癌信号通路网络。
(注：上述文献为虚构示例，实际文献需通过PubMed或SciHub等学术平台检索。)
MYBL1. a member of the MYB family of transcription factors, plays a critical role in regulating cell cycle progression, differentiation, and apoptosis. It contains a conserved N-terminal DNA-binding domain that recognizes specific promoter sequences, enabling transcriptional activation or repression of target genes. MYBL1 (also called A-MYB) is predominantly expressed in proliferating cells and has been implicated in hematopoietic development, spermatogenesis, and neuronal function. Dysregulation of MYBL1 is associated with cancers, particularly leukemias and lymphomas, where its overexpression promotes tumorigenesis by enhancing cell survival and proliferation pathways.
Recombinant human MYBL1 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems to study its biochemical properties and interactions. Purification methods often involve affinity chromatography tags (e.g., His-tag) for high-yield recovery. This engineered protein serves as a vital tool for *in vitro* assays, including DNA-binding studies (e.g., EMSA), protein-protein interaction analyses, and screening for inhibitors targeting MYBL1 activity. Researchers also utilize it to investigate post-translational modifications (e.g., phosphorylation) that modulate its transcriptional activity. Its recombinant form enables precise exploration of MYBL1’s role in gene regulatory networks and potential therapeutic targeting in malignancies.
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