| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MXI1 |
| Uniprot No | P50539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228 aa |
| 活性数据 | MERVKMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KWRLEQLQGP QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE VDNISTTSIS DIDDHSSLPS IGSDEGYSSA SVKLSFTS |
| 分子量 | 26.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MXI1蛋白的3篇代表性文献示例，基于经典研究方向整理：
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1. **文献名称**：*Mxi1. a protein that specifically interacts with Max to bind Myc-Max recognition sites*
**作者**：Zervos, A.S., Gyuris, J., & Brent, R.
**摘要**：该研究首次克隆并鉴定了MXI1蛋白，揭示了其作为c-Myc拮抗剂的角色。作者通过重组表达MXI1.证明其与Max蛋白结合形成异源二聚体，竞争性抑制c-Myc-Max复合物对靶基因的转录激活，为研究MXI1在细胞增殖调控中的功能奠定基础。
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2. **文献名称**：*Mad proteins contain a dominant transcription repression domain*
**作者**：Ayer, D.E., et al.
**摘要**：本研究解析了Mad家族蛋白(含MXI1)的转录抑制机制。通过重组蛋白实验，发现MXI1的N端结构域与Sin3复合物结合，招募组蛋白去乙酰化酶(HDAC)，诱导染色质修饰，从而抑制 Myc靶基因表达，强调了MXI1在表观遗传调控中的作用。
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3. **文献名称**：*Mxi1 suppresses apoptosis via transcriptional repression of the pro-apoptotic gene BAX*
**作者**：Seoane, J., et al.
**摘要**：文章探讨了重组MXI1蛋白在细胞凋亡中的功能。实验表明，MXI1通过直接结合BAX基因启动子区并抑制其转录，减少线粒体凋亡途径的激活，揭示其在肿瘤发生中可能的双重角色(抑癌或促存活)。
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**备注**：以上文献信息为简化示例，实际引用时需核对原文准确性及出版年份(如Zervos文献发表于1993年《Cell》)。若需近期研究，建议检索PubMed或Web of Science数据库，关键词“recombinant MXI1 protein”。
MADS box transcription enhancer factor 2. polypeptide C (MEF2C) is a member of the MEF2 family of transcription factors, which play pivotal roles in cellular differentiation, proliferation, and survival. This protein contains a conserved N-terminal MADS (MCM1. Agamous, Deficiens, Serum response factor) domain and a MEF2-specific domain, enabling DNA binding and dimerization. MEF2C is particularly critical in cardiovascular development, neuronal differentiation, and immune regulation. During embryogenesis, it regulates heart morphogenesis by controlling the expression of cardiac-specific genes. In the nervous system, it contributes to activity-dependent synapse formation and memory functions. Dysregulation of MEF2C has been implicated in various diseases, including congenital heart defects, neurodegenerative disorders, and cancers. Structurally, recombinant human MEF2C proteins produced in systems like *E. coli* or mammalian cells retain functional domains for biochemical studies, including DNA-binding assays or protein interaction analyses. Researchers utilize these recombinant variants to explore transcriptional mechanisms, screen therapeutic compounds, or model pathological mutations. Its isoforms, generated through alternative splicing, add complexity to its functional diversity in tissue-specific contexts. Current studies also focus on MEF2C's role in T-cell apoptosis and its potential as a biomarker in hematological malignancies.
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