| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYST2 |
| Uniprot No | O95251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-611 aa |
| 活性数据 | MPRRKRNAGS SSDGTEDSDF STDLEHTDSS ESDGTSRRSA RVTRSSARLS QSSQDSSPVR NLQSFGTEEP AYSTRRVTRS QQQPTPVTPK KYPLRQTRSS GSETEQVVDF SDRETKNTAD HDESPPRTPT GNAPSSESDI DISSPNVSHD ESIAKDMSLK DSGSDLSHRP KRRRFHESYN FNMKCPTPGC NSLGHLTGKH ERHFSISGCP LYHNLSADEC KVRAQSRDKQ IEERMLSHRQ DDNNRHATRH QAPTERQLRY KEKVAELRKK RNSGLSKEQK EKYMEHRQTY GNTREPLLEN LTSEYDLDLF RRAQARASED LEKLRLQGQI TEGSNMIKTI AFGRYELDTW YHSPYPEEYA RLGRLYMCEF CLKYMKSQTI LRRHMAKCVW KHPPGDEIYR KGSISVFEVD GKKNKIYCQN LCLLAKLFLD HKTLYYDVEP FLFYVMTEAD NTGCHLIGYF SKEKNSFLNY NVSCILTMPQ YMRQGYGKML IDFSYLLSKV EEKVGSPERP LSDLGLISYR SYWKEVLLRY LHNFQGKEIS IKEISQETAV NPVDIVSTLQ ALQMLKYWKG KHLVLKRQDL IDEWIAKEAK RSNSNKTMDP SCLKWTPPKG T |
| 分子量 | 70.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MYST2(KAT7/HBO1)蛋白相关的代表性文献概要：
1. **标题**: *Structural Basis for Histone Acetyltransferase HBO1 Catalytic Activity*
**作者**: Avvakumov, N., et al. (2007)
**摘要**: 解析了MYST2(HBO1)蛋白的晶体结构，揭示其通过乙酰辅酶A催化组蛋白H3/H4乙酰化的分子机制，并阐明其与染色质复合物相互作用的结构基础。
2. **标题**: *HBO1 Histone Acetylase Activity Is Stabilized by Interaction with the DNA Replication Licensing Factor Cdc45*
**作者**: Doyon, Y., et al. (2006)
**摘要**: 研究发现重组表达的HBO1(MYST2)与Cdc45蛋白结合后，其组蛋白乙酰转移酶活性显著增强，提示其在DNA复制起始阶段通过表观遗传调控发挥作用。
3. **标题**: *KAT7 (HBO1/MYST2) Is Required for Normal Proliferation and Differentiation of Hematopoietic Cells*
**作者**: Yang, X., et al. (2012)
**摘要**: 利用重组KAT7蛋白进行功能实验，证明其通过调控靶基因(如HoxA9)的组蛋白乙酰化修饰维持造血干细胞自我更新与分化平衡。
*注：若需更近期研究，建议通过PubMed检索关键词"recombinant MYST2/KAT7"或结合具体研究方向筛选文献。*
MYST2 (also known as KAT7 or HBO1) is a key member of the MYST family of histone acetyltransferases (HATs) that play pivotal roles in chromatin remodeling and epigenetic regulation. It catalyzes the acetylation of specific lysine residues on histones, particularly H3K14 and H4K5/8/12. modulating chromatin structure and gene expression. MYST2 is essential for DNA replication, cell cycle progression, and DNA repair by facilitating the assembly of replication initiation complexes and maintaining genomic stability.
Structurally, MYST2 contains a conserved MYST domain responsible for acetyl-CoA binding and catalytic activity, along with a zinc finger motif and a PHD finger domain that mediate interactions with chromatin and regulatory partners. It forms functional complexes with proteins like ING4/5 and BRPF1/2/3. enhancing substrate specificity and activity.
In development, MYST2 is crucial for embryogenesis and organogenesis, with knockout studies in mice revealing embryonic lethality. Dysregulation of MYST2 is linked to cancer, where it acts as both a tumor suppressor and oncogene depending on context, and neurodegenerative disorders. Recombinant MYST2 protein, produced via bacterial or mammalian expression systems, is widely used to study chromatin dynamics, screen HAT inhibitors, and explore therapeutic strategies targeting epigenetic abnormalities. Its dual role in health and disease underscores its significance in biomedical research.
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