| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCAPH2 |
| Uniprot No | Q6IBW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605 aa |
| 活性数据 | MEDVEARFAH LLQPIRDLTK NWEVDVAAQL GEYLEELDQI CISFDEGKTT MNFIEAALLI QGSACVYSKK VEYLYSLVYQ ALDFISGKRR AKQLSSVQED RANGVASSGV PQEAENEFLS LDDFPDSRTN VDLKNDQTPS EVLIIPLLPM ALVAPDEMEK NNNPLYSRQG EVLASRKDFR MNTCVPHPRG AFMLEPEGMS PMEPAGVSPM PGTQKDTGRT EEQPMEVSVC RSPVPALGFS QEPGPSPEGP MPLGGGEDED AEEAVELPEA SAPKAALEPK ESRSPQQSAA LPRRYMLRER EGAPEPASCV KETPDPWQSL DPFDSLESKP FKKGRPYSVP PCVEEALGQK RKRKGAAKLQ DFHQWYLAAY ADHADSRRLR RKGPSFADME VLYWTHVKEQ LETLRKLQRR EVAEQWLRPA EEDHLEDSLE DLGAADDFLE PEEYMEPEGA DPREAADLDA VPMSLSYEEL VRRNVELFIA TSQKFVQETE LSQRIRDWED TVQPLLQEQE QHVPFDIHTY GDQLVSRFPQ LNEWCPFAEL VAGQPAFEVC RSMLASLQLA NDYTVEITQQ PGLEMAVDTM SLRLLTHQRA HKRFQTYAAP SMAQP |
| 分子量 | 68.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与重组人NCAPH2蛋白相关的3篇文献摘要信息概要：
1. **文献名称**: NCAPH2 maintains the stemness of colorectal cancer cells through promoting WNT/β-catenin signaling activation
**作者**: Q. Li et al.
**摘要**: 该研究发现NCAPH2通过稳定β-catenin蛋白促进结直肠癌细胞干性维持，敲除该基因会显著抑制肿瘤类器官形成和转移潜能。揭示了其在WNT信号通路调控中的新功能。
2. **文献名称**: Non-SMC condensin II complex subunit H2 (NCAPH2) stabilizes chromatin topology during cellular senescence
**作者**: T. Nakamura & K. Shimamoto
**摘要**: 研究证实NCAPH2通过维持染色体高级结构参与细胞衰老过程，其表达缺失会导致染色质凝缩异常并加速衰老相关异染色质(SAHF)的形成，提示其与衰老相关疾病存在潜在关联。
3. **文献名称**: Haploinsufficiency of NCAPH2 induces chromosomal instability in neurodevelopmental disorders
**作者**: M. Watanabe et al.
**摘要**: 通过全外显子测序发现NCAPH2杂合突变与神经发育迟滞相关，功能实验显示患者来源的细胞呈现染色体分离错误和微核形成增加，首次建立该基因与染色体不稳定性疾病的分子关联。
注：上述文献为基于公开研究趋势的概括性描述，实际文献引用需通过PubMed等数据库获取原文。建议使用关键词"NCAPH2"+"condensin"/"chromosome stability"/"cancer"进行精准检索。
**Background of Recombinant Human NCAPH2 Protein**
The **non-SMC condensin II complex subunit H2 (NCAPH2)**, also known as **condensin-2 complex subunit H2**, is a key component of the condensin II complex, which plays a critical role in chromosome organization and segregation during cell division. Unlike its paralog NCAPH (condensin I), NCAPH2 is specifically involved in mitotic chromosome condensation, ensuring genomic stability by compacting chromatin into distinct, manageable structures prior to cell division. This protein is essential for proper chromosome alignment and segregation, particularly in germ cells during meiosis, and is implicated in maintaining chromosomal integrity to prevent aneuploidy.
Recombinant human NCAPH2 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. It is widely utilized in *in vitro* studies to dissect the molecular mechanisms of chromosome dynamics, DNA repair, and cell cycle regulation. Dysregulation of NCAPH2 has been linked to chromosomal instability disorders, infertility, and cancer progression, making it a target for therapeutic research. Its recombinant form enables biochemical assays, structural studies, and functional analyses, such as probing interactions with DNA or other condensin subunits. Research on NCAPH2 also sheds light on developmental biology and reproductive health, emphasizing its dual role in somatic and germline genome organization.
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