| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCAPG2 |
| Uniprot No | Q86XI2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-935 aa |
| 活性数据 | MLLECFININYIKKEEGRRFLSCLFNWNINFIKMIHGTIKNQLQGLQKSLMVYIAEIYFRAWKKASGKILEAIENDCIQDFMFHGIHLPRRSPVHSKVREVLSYFHHQKKVRQGVEEMLYRLYKPILWRGLKARNSEVRSNAALLFVEAFPIRDPNLHAIEMDSEIQKQFEELYSLLEDPYPMVRSTGILGVCKITSKYWEMMPPTILIDLLKKVTGELAFDTSSADVRCSVFKCLPMILDNKLSHPLLEQLLPALRYSLHDNSEKVRVAFVDMLLKIKAVRAAKFWKICPMEHILVRLETDSRPVSRRLVSLIFNSFLPVNQPEEVWCERCVTLVQMNHAAARRFYQYAHEHTACTNIAKLIHVIRHCLNACIQRAVREPPEDEEEEDGREKENVTVLDKTLSVNDVACMAGLLEIIVILWKSIDRSMENNKEAKLYTINKFASVLPEYLKVFKDDRCKIPLFMLMSFMPASAVPPFSCGVISTLRSREEGAVDKSYCTLLDCLCSWGQVGHILELVDNWLPTEHAQAKSNTASKGRVQIHDTRPVKPELALVYIEYLLTHPKNRECLLSAPRKKLNHLLKALETSKADLESLLQTPGGKPRGFSEAAAPRAFGLHCRLSIHLQHKFCSEGKVYLSMLEDTGFWLESKILSFIQDQEEDYLKLHRVIYQQIIQTYLTVCKDVVMVGLGDHQFQMQLLQRSLGIMQTVKGFFYVSLLLDILKEITGSSLIQKTDSDEEVAMLLDTVQKVFQKMLECIARSFRKQPEEGLRLLYSVQRPLHEFITAVQSRHTDTPVHRGVLSTLIAGPVVEISHQLRKVSDVEELTPPEHLSDLPPFSRCLIGIIIKSSNVVRSFLDELKACVASNDIEGIVCLTAAVHIILVINAGKHKSSKVREVAATVHRKLKTFMEITLEEDSIERFLYESSSRTLGELLNS |
| 分子量 | 133.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NCAPG2蛋白的3-4篇参考文献示例，涵盖其功能、表达及疾病关联研究：
1. **文献名称**：*"NCAPG2 promotes hepatocellular carcinoma progression by regulating cell cycle and epithelial-mesenchymal transition"*
**作者**：Wang L, et al.
**摘要**：探讨NCAPG2在肝癌中的高表达及其促癌机制，重组蛋白实验表明其通过调控细胞周期蛋白(如Cyclin B1)和EMT通路促进肿瘤侵袭转移。
2. **文献名称**：*"Recombinant expression and functional characterization of human NCAPG2 in chromosome segregation"*
**作者**：Smith J, et al.
**摘要**：描述重组人NCAPG2蛋白在大肠杆菌中的表达与纯化，并验证其在体外染色体分离中的作用，揭示其与condensin复合体其他亚基的相互作用。
3. **文献名称**：*"NCAPG2 as a novel biomarker in gastric cancer: recombinant protein-based functional studies"*
**作者**：Chen H, et al.
**摘要**：通过重组NCAPG2蛋白的过表达实验，证明其抑制胃癌细胞凋亡并加速增殖，提示其作为治疗靶点的潜力。
4. **文献名称**：*"Structural analysis of recombinant human NCAPG2 reveals its role in chromatin condensation"*
**作者**：Kim S, et al.
**摘要**：利用重组蛋白的X射线晶体学解析NCAPG2的ATP酶结构域，阐明其在染色质凝聚过程中维持基因组稳定的分子机制。
(注：以上为假设性参考文献，实际研究中建议通过PubMed或SciFinder等数据库检索真实文献。)
The non-SMC condensin II complex subunit G2 (NCAPG2) is a critical component of the condensin II protein complex, which plays essential roles in chromosome organization and segregation during mitosis. As part of the conserved condensin family, NCAPG2 contributes to chromatin condensation, sister chromatid resolution, and mitotic chromosome architecture by regulating DNA topology through ATP-dependent interactions. Structurally, it interacts with other condensin II subunits (NCAPH2. NCAPD3. SMC2. SMC4) to form a ring-like structure that stabilizes higher-order chromosomal folding. Dysregulation of NCAPG2 is linked to genomic instability and mitotic errors, with overexpression observed in multiple cancers, including hepatocellular carcinoma and breast cancer, where it correlates with poor prognosis and aggressive phenotypes. Recombinant NCAPG2 protein, typically produced in E. coli or mammalian expression systems, enables functional studies of its role in cell cycle regulation, DNA repair, and epigenetic modulation. Its recombinant form is valuable for investigating post-translational modifications (e.g., phosphorylation by CDK1), chromatin-binding dynamics, and interactions with regulatory partners. Current research explores NCAPG2's potential as a diagnostic marker or therapeutic target, though challenges remain in elucidating its precise mechanistic contributions to chromosomal pathologies and cancer progression.
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