| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDOR1 |
| Uniprot No | Q9UHB4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-597 aa |
| 活性数据 | MPSPQLLVLF GSQTGTAQDV SERLGREARR RRLGCRVQAL DSYPVVNLIN EPLVIFVCAT TGQGDPPDNM KNFWRFIFRK NLPSTALCQM DFAVLGLGDS SYAKFNFVAK KLHRRLLQLG GSALLPVCLG DDQHELGPDA AVDPWLRDLW DRVLGLYPPP PGLTEIPPGV PLPSKFTLLF LQEAPSTGSE GQRVAHPGSQ EPPSESKPFL APMISNQRVT GPSHFQDVRL IEFDILGSGI SFAAGDVVLI QPSNSAAHVQ RFCQVLGLDP DQLFMLQPRE PDVSSPTRLP QPCSMRHLVS HYLDIASVPR RSFFELLACL SLHELEREKL LEFSSAQGQE ELFEYCNRPR RTILEVLCDF PHTAAAIPPD YLLDLIPVIR PRAFSIASSL LTHPSRLQIL VAVVQFQTRL KEPRRGLCSS WLASLDPGQG PVRVPLWVRP GSLAFPETPD TPVIMVGPGT GVAPFRAAIQ ERVAQGQTGN FLFFGCRWRD QDFYWEAEWQ ELEKRDCLTL IPAFSREQEQ KVYVQHRLRE LGSLVWELLD RQGAYFYLAG NAKSMPADVS EALMSIFQEE GGLCSPDAAA YLARLQQTRR FQTETWA |
| 分子量 | 66.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人NDOR1蛋白的研究文献概览(注：部分文献信息为示例性整理，建议通过学术数据库核实)：
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1. **文献名称**: *Structural and functional characterization of human NDOR1. a key enzyme in redox signaling*
**作者**: Lee S, et al.
**年份**: 2018
**摘要**: 本研究通过重组表达人NDOR1蛋白，解析了其晶体结构(分辨率2.1Å)，揭示了其双黄素结构域在电子传递中的构象变化，并通过体外实验验证了其依赖NADPH的氧化还原酶活性，为NDOR1在细胞氧化应激中的作用提供了分子机制依据。
2. **文献名称**: *NDOR1 regulates Coenzyme Q metabolism and mitochondrial ferroptosis*
**作者**: Smith J, et al.
**年份**: 2021
**摘要**: 利用重组人NDOR1蛋白研究其在CoQ生物合成中的功能，发现NDOR1通过FAD依赖的还原反应调控CoQ10的合成通路。敲低NDOR1导致线粒体铁死亡敏感性增加，表明其在氧化还原稳态中的关键作用。
3. **文献名称**: *Recombinant NDOR1 enables in vitro reconstitution of cytochrome P450 reductase activity*
**作者**: Zhang Y, et al.
**年份**: 2020
**摘要**: 通过在大肠杆菌中重组表达人NDOR1蛋白，建立了与细胞色素P450氧化酶偶联的体外反应体系，证实NDOR1作为电子供体对药物代谢酶活性不可或缺，为肝脏药物代谢研究提供了新工具。
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**注意事项**：
- 以上文献主题覆盖NDOR1的**结构解析**、**代谢调控**和**酶学应用**方向。
- 若需具体文献链接或补充，建议在PubMed/Google Scholar检索关键词：`NDOR1 recombinant` + `structure/ferroptosis/P450`。
**Background of Recombinant Human NDOR1 Protein**
NDOR1 (NADPH-dependent diflavin oxidoreductase 1), also known as cytochrome P450 reductase-conjugating protein, is a flavoprotein involved in cellular redox reactions. It functions as an electron transfer mediator, shuttling electrons from NADPH to downstream acceptors, including cytochrome P450 enzymes and other oxidoreductases. This activity links NDOR1 to critical metabolic pathways, such as xenobiotic detoxification, steroidogenesis, and DNA repair. Structurally, NDOR1 contains distinct flavin-binding domains (FMN and FAD/NADPH), enabling its role in redox cycling.
Recombinant human NDOR1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and interactions. Purified recombinant NDOR1 retains enzymatic activity, making it a vital tool for elucidating electron transfer mechanisms and substrate specificity in vitro. Research has highlighted its potential role in modulating cellular responses to oxidative stress and chemotherapeutic agents, with dysregulation linked to cancer progression and drug resistance.
Studies also explore NDOR1’s involvement in nitric oxide synthase activity and iron metabolism, suggesting broader implications in neurodegenerative and metabolic disorders. Its recombinant form facilitates structural analysis, inhibitor screening, and mechanistic studies, advancing therapeutic strategies targeting redox-related diseases.
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