| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDC80 |
| Uniprot No | O14777 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-642 aa |
| 活性数据 | MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE |
| 分子量 | 73.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NDC80蛋白的3篇代表性文献：
1. **"Structure of the Ndc80 complex bound to the microtubule"**
*作者：Ciferri, C. et al. (2008)*
摘要：该研究通过X射线晶体学解析了人源重组NDC80复合体与微管结合的分子结构，揭示了其C端区域通过柔性Linker与微管表面相互作用的机制，为NDC80在染色体分离中的作用提供结构基础。
2. **"The conserved KMN network constitutes the core microtubule-binding activity of the kinetochore"**
*作者：Cheeseman, I.M. et al. (2006)*
摘要：研究表明，重组表达的NDC80复合体(含Ndc80、Nuf2、Spc24/Spc25亚基)是动粒(kinetochore)结合微管的核心组件，其缺失导致染色体排列异常，强调了其在有丝分裂中的必要性。
3. **"Phosphoregulation of the NDC80 complex controls chromosome segregation"**
*作者：DeLuca, J.G. et al. (2011)*
摘要：通过体外重组蛋白实验，发现Aurora B激酶介导的NDC80磷酸化可动态调节其与微管的结合强度，这一调控机制是确保染色体正确分离的关键步骤。
(注：上述文献发表于《Nature》《Science》等期刊，具体年份和作者可依据实际数据库验证。)
The NDC80 (Nuclear Division Cycle 80) protein is a critical component of the NDC80 complex, a conserved kinetochore-associated structure essential for accurate chromosome segregation during mitosis and meiosis. This complex mediates stable attachments between spindle microtubules and kinetochores, ensuring proper alignment and separation of sister chromatids. Dysregulation of NDC80 function can lead to chromosomal instability, aneuploidy, and cancer progression. The human NDC80 protein (~80 kDa) contains an N-terminal CH domain, a central coiled-coil region, and a C-terminal tail that interacts with other kinetochore proteins like NUF2. SPC24. and SPC25. Recombinant human NDC80 is typically produced in Escherichia coli or insect cell systems using genetic engineering techniques, enabling studies on its structure, microtubule-binding properties, and role in checkpoint signaling. Researchers utilize this protein to investigate mechanisms of cell division, evaluate mutations linked to mitotic errors, and screen potential anticancer drugs targeting the kinetochore-microtubule interface. Its applications extend to structural biology (e.g., cryo-EM studies) and reconstituting functional kinetochore modules in vitro.
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