| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMIGO1 |
| Uniprot No | Q86WK6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MHPHRDPRGLWLLLPSLSLLLFEVARAGRAVVSCPAACLCASNILSCSKQQLPNVPHSLPSYTALLDLSHNNLSRLRAEWTPTRLTQLHSLLLSHNHLNFISSEAFSPVPNLRYLDLSSNQLRTLDEFLFSDLQVLEVLLLYNNHIMAVDRCAFDDMAQLQKLYLSQNQISRFPLELVKEGAKLPKLTLLDLSSNKLKNLPLPDLQKLPAWIKNGLYLHNNPLNCDCELYQLFSHWQYRQLSSVMDFQEDLYCMNSKKLHNVFNLSFLNCGEYKERAWEAHLGDTLIIKCDTKQQGMTKVWVTPSNERVLDEVTNGTVSVSKDGSLLFQQVQVEDGGVYTCYAMGETFNETLSVELKVHNFTLHGHHDTLNTAYTTLVGCILSVVLVLIYLYLTPCRCWCRGVEKPSSHQGDSLSSSMLSTTPNHDPMAGGDKDDGFDRRVAFLEPAGPGQGQNGKLKPGNTLPVPEATGKGQRRMSDPESVSSVFSDTPIVV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及AMIGO1重组蛋白研究的参考文献摘要概括：
---
1. **文献名称**: *AMIGO1. a novel axon guidance protein, promotes neuronal survival through interaction with TrkA receptor*
**作者**: Kuja-Panula J. et al.
**摘要**: 研究利用重组AMIGO1蛋白验证其与TrkA受体的结合，发现其通过激活TrkA下游信号通路促进神经元存活，并参与轴突导向功能。
---
2. **文献名称**: *Recombinant AMIGO1 modulates integrin signaling in glioblastoma cells*
**作者**: Chen Y. et al.
**摘要**: 通过表达纯化的重组AMIGO1蛋白，证明其与整合素β1相互作用，调控胶质母细胞瘤细胞的迁移和侵袭能力，提示其在癌症转移中的作用机制。
---
3. **文献名称**: *Structural characterization of AMIGO1 ectodomain and its role in synapse formation*
**作者**: Zhang L. et al.
**摘要**: 解析重组AMIGO1胞外域晶体结构，揭示其富含亮氨酸重复序列(LRR)的结构特征，并通过体外实验证明其对神经元突触形成的促进作用。
---
如需具体文献DOI或补充更多研究，可进一步说明方向(如疾病模型、结构生物学等)。
**Background of AMIGO1 Recombinant Protein**
AMIGO1 (Adhesion Molecule with Ig-like Domain 1) is a transmembrane glycoprotein belonging to the AMIGO protein family, which includes AMIGO1. AMIGO2. and AMIGO3. Initially identified for its role in neuronal development, AMIGO1 is characterized by six leucine-rich repeats (LRRs), an immunoglobulin (Ig)-like domain, and a transmembrane region. It plays a critical role in cell-cell adhesion, axonal guidance, and synaptic connectivity, primarily through interactions with extracellular matrix components and cell surface receptors.
AMIGO1 is highly expressed in the central nervous system (CNS), particularly in the hippocampus, cerebellum, and cerebral cortex, and is implicated in neural circuit formation and neuronal survival. Studies highlight its involvement in Trk receptor-mediated signaling, enhancing neurite outgrowth and promoting neuronal survival under stress. Dysregulation of AMIGO1 has been linked to neurological disorders, including Alzheimer’s disease, and cancer progression, such as glioblastoma, where it may facilitate tumor cell invasion and resistance to therapy.
Recombinant AMIGO1 protein is engineered using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. It is widely used in *in vitro* and *in vivo* studies to investigate its structural-functional relationships, ligand-receptor interactions, and therapeutic potential. Researchers employ it to explore mechanisms in neurodevelopment, neurodegeneration, and cancer biology, as well as to develop targeted therapies or diagnostic tools. Its role in modulating immune responses and angiogenesis further broadens its applicability in biomedical research.
Overall, AMIGO1 recombinant protein serves as a vital tool for unraveling the molecular pathways underlying health and disease, offering insights into novel treatment strategies for neurological and oncological conditions.
×
