| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IK |
| Uniprot No | Q13123 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-557aa |
| 氨基酸序列 | MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNVYRMLFKSKAYERNELFLPGRMAYVVDLDDEYADTDIPTTLIRSKADCPTMEAQTTLTTNDIVISKLTQILSYLRQGTRNKKLKKKDKGKLEEKKPPEADMNIFEDIGDYVPSTTKTPRDKERERYRERERDRERDRDRDRERERERDRERERERDREREEEKKRHSYFEKPKVDDEPMDVDKGPGSTKELIKSINEKFAGSAGWEGTESLKKPEDKKQLGDFFGMSNSYAECYPATMDDMAVDSDEEVDYSKMDQGNKKGPLGRWDFDTQEEYSEYMNNKEALPKAAFQYGIKMSEGRKTRRFKETNDKAELDRQWKKISAIIEKRKKMEADGVEVKRPKY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IK(IκB激酶)重组蛋白的3篇参考文献及其摘要概括：
1. **《Recombinant IKKβ Production and Its Role in NF-κB Activation》**
- 作者：Chen, L., et al.
- 摘要：研究通过大肠杆菌系统高效表达并纯化具有活性的重组IKKβ蛋白，证实其在体外可磷酸化IκBα并激活NF-κB信号通路，为炎症机制研究提供工具。
2. **《Structural Insights into IK Kinase Complex Assembly Using Recombinant Protein Co-expression》**
- 作者：Miller, S., & Yamamoto, H.
- 摘要：利用昆虫细胞共表达系统制备IKKα/IKKγ复合物，通过冷冻电镜解析其三维结构，揭示激酶活性调控机制及潜在药物靶点。
3. **《Application of Recombinant IKK in High-Throughput Drug Screening for Anti-Cancer Therapeutics》**
- 作者：Gupta, R., et al.
- 摘要：开发基于重组IKK蛋白的体外激酶活性检测体系，用于筛选靶向NF-κB通路的小分子抑制剂，发现多个候选化合物可抑制肿瘤细胞增殖。
注：以上文献信息为示例性内容，实际引用需以具体论文为准。若需真实文献，建议通过PubMed或Web of Science以关键词“IkappaB kinase recombinant”检索。
**Background of IK Recombinant Proteins**
IK recombinant proteins, often associated with the IκB kinase (IKK) family, are engineered molecules central to studying cellular signaling pathways, particularly the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) pathway. The IKK complex, comprising catalytic subunits (IKKα/IKK1 and IKKβ/IKK2) and a regulatory subunit (IKKγ/NEMO), regulates NF-κB activation by phosphorylating inhibitory IκB proteins, leading to their degradation and subsequent NF-κB nuclear translocation. This pathway is critical in immune responses, inflammation, apoptosis, and cell survival.
Recombinant IK proteins are produced via genetic engineering, enabling precise study of their structure, function, and interactions. Researchers utilize expression systems (e.g., bacterial, mammalian) to generate these proteins, often tagging them for purification or tracking. Key applications include elucidating kinase-substrate dynamics, screening inhibitors for therapeutic development (e.g., targeting inflammatory diseases or cancer), and reconstituting signaling cascades *in vitro*.
The development of IK recombinant proteins has addressed challenges in native protein isolation, such as low abundance and instability. Mutant variants (e.g., kinase-dead or constitutively active forms) further dissect mechanistic roles in disease models. For instance, IKKβ inhibitors are explored for chronic inflammation, while IKKα is linked to cancer metastasis.
Despite advancements, challenges persist, including maintaining post-translational modifications in recombinant forms and achieving tissue-specific targeting *in vivo*. Ongoing research focuses on optimizing expression systems and leveraging structural biology (e.g., cryo-EM) to refine drug design. Overall, IK recombinant proteins remain pivotal in decoding NF-κB biology and advancing targeted therapies.
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