| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOVA2 |
| Uniprot No | Q9UNW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492 aa |
| 活性数据 | MEPEAPDSRK RPLETPPEVV CTKRSNTGEE GEYFLKVLIP SYAAGSIIGK GGQTIVQLQK ETGATIKLSK SKDFYPGTTE RVCLVQGTAE ALNAVHSFIA EKVREIPQAM TKPEVVNILQ PQTTMNPDRA KQAKLIVPNS TAGLIIGKGG ATVKAVMEQS GAWVQLSQKP EGINLQERVV TVSGEPEQVH KAVSAIVQKV QEDPQSSSCL NISYANVAGP VANSNPTGSP YASPADVLPA AAAASAAAAS GLLGPAGLAG VGAFPAALPA FSGTDLLAIS TALNTLASYG YNTNSLGLGL NSAAASGVLA AVAAGANPAA AAAANLLASY AGEAGAGPAG GAAPPPPPPP GALGSFALAA AANGYLGAGA GGGAGGGGGP LVAAAAAAGA AGGFLTAEKL AAESAKELVE IAVPENLVGA ILGKGGKTLV EYQELTGARI QISKKGEFLP GTRNRRVTIT GSPAATQAAQ YLISQRVTYE QGVRASNPQK VG |
| 分子量 | 49.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOVA2蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"NOVA2-mediated RNA regulation is required for axonal pathfinding during mammalian brain development"*
**作者**: Saito Y, Miranda-Rottmann S, Ruggiu M, Park CY, Fak JJ, Zhong R, et al.
**摘要**: 本研究通过重组人NOVA2蛋白验证其在神经元轴突导向中的作用，发现NOVA2通过调控下游mRNA的剪接和稳定性，影响轴突生长锥的响应信号，从而调控小鼠大脑皮层神经元的迁移路径。
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2. **文献名称**: *"Structural basis of RNA recognition and dimerization by the STAR proteins Quaking and Nova2"*
**作者**: Teplova M, Song J, Gaw HY, Farazi TA, Tuschl T, Patel DJ.
**摘要**: 该研究解析了重组人NOVA2蛋白的RNA结合结构域晶体结构，揭示其通过二聚化特异性识别靶RNA序列的机制，并阐明NOVA2与神经疾病相关突变导致RNA结合能力缺陷的分子基础。
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3. **文献名称**: *"Recombinant NOVA2 protein rescues synaptic deficits in a mouse model of autism spectrum disorder"*
**作者**: Lee A, Wang J, Bagni C, Zukin RS.
**摘要**: 利用重组NOVA2蛋白注射至自闭症模型小鼠海马区，发现其可逆转突触蛋白异常剪接，改善神经元突触可塑性和认知功能，为NOVA2相关的RNA代谢失调与神经发育障碍的关联提供治疗依据。
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注：以上文献信息为综合领域知识生成的示例，若需真实文献，建议通过PubMed或Google Scholar以“recombinant NOVA2 protein”或“NOVA2 RNA binding”等关键词检索。
**Background of Recombinant Human NOVA2 Protein**
Recombinant human NOVA2 (Neuro-oncological ventral antigen 2) is a RNA-binding protein belonging to the NOVA family of neuronal splicing regulators. It is primarily expressed in the central nervous system and plays a critical role in regulating alternative splicing of pre-mRNAs, which is essential for neuronal development, synaptic plasticity, and function. NOVA2 contains tandem K-homology (KH) domains that enable sequence-specific interactions with RNA, particularly within intronic regions of target transcripts.
NOVA2 is implicated in diverse neurological processes, including axon guidance, synapse formation, and neural circuit assembly. Studies have linked its dysfunction to neurodevelopmental disorders and neurodegenerative diseases. The recombinant form of NOVA2 is typically produced in *E. coli* or mammalian expression systems, allowing researchers to study its RNA-binding properties, structural dynamics, and interactions with splicing machinery *in vitro*.
Its role as a splicing regulator has made NOVA2 a valuable tool for investigating post-transcriptional gene regulation in neurobiology. Recombinant NOVA2 is also utilized in functional assays, structural studies, and therapeutic research aimed at modulating RNA-processing defects in neurological conditions.
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