| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOLA2 |
| Uniprot No | Q9NX24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153 aa |
| 活性数据 | MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL |
| 分子量 | 17.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOLA2(NHP2)蛋白的3篇文献示例，内容基于相关领域的研究方向进行模拟整合：
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1. **文献名称**：*Expression and Purification of Recombinant Human NOLA2 for Structural Studies*
**作者**：Smith A, et al.
**摘要**：该研究报道了人NOLA2蛋白在大肠杆菌中的重组表达及纯化方法，采用亲和层析技术获得高纯度蛋白。通过X射线晶体学解析了NOLA2的三维结构，揭示了其在H/ACA核糖核蛋白复合体中的保守结构域，为研究其与RNA的相互作用提供了基础。
2. **文献名称**：*Functional Analysis of NOLA2 in Telomerase RNA Stability*
**作者**：Johnson R, et al.
**摘要**：本文探讨了重组NOLA2蛋白在端粒酶复合体中的功能，发现其通过与端粒酶RNA(TERC)结合维持RNA稳定性。通过体外重组蛋白实验证明，NOLA2缺失会导致TERC降解并抑制端粒酶活性，提示其在细胞衰老和癌症中的潜在作用。
3. **文献名称**：*NOLA2 Mutations and Dyskeratosis Congenita: Biochemical Characterization Using Recombinant Protein*
**作者**：Lee H, et al.
**摘要**：研究利用重组表达的NOLA2蛋白，分析了遗传性疾病先天性角化不良相关突变的分子机制。实验表明，突变体NOLA2丧失与核心蛋白GAR1的结合能力，导致H/ACA复合体组装缺陷，进而影响核糖体RNA加工和细胞增殖。
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**备注**：以上为模拟参考文献，实际文献需通过PubMed、Web of Science等平台检索确认。建议使用关键词“NHP2 recombinant”、“NOLA2 expression”或“H/ACA RNP complex”查找最新研究。
**Background of Recombinant Human NOLA2 Protein**
Recombinant human NOLA2 (Nucleolar protein 2), also known as WDR3 (WD repeat domain 3), is a conserved eukaryotic protein critical for ribosome biogenesis. Structurally, NOLA2 contains WD40 repeats, which mediate protein-protein interactions, facilitating its role in pre-rRNA processing and 18S rRNA maturation. It localizes to the nucleolus, coordinating with other ribosome assembly factors (e.g., NOP56 and NOP58) to ensure proper small ribosomal subunit formation. Dysregulation of NOLA2 is linked to developmental disorders, cancer progression, and neurodegeneration, reflecting its essentiality in cellular proliferation and homeostasis.
The recombinant form is produced via expression systems (e.g., *E. coli* or mammalian cells*) using genetic engineering, enabling high purity and consistent yield for research. Recombinant NOLA2 aids in studying ribosomopathies, rRNA processing mechanisms, and drug screening targeting nucleolar pathways. Its applications extend to investigating cellular stress responses, as disrupted ribosome biogenesis activates p53-dependent checkpoints. Overall, recombinant NOLA2 serves as a vital tool for dissecting ribosome assembly dynamics and its implications in human diseases.
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