| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOXO1 |
| Uniprot No | Q8NFA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376 aa |
| 活性数据 | MAGPRYPVSV QGAALVQIKR LQTFAFSVRW SDGSDTFVRR SWDEFRQLKK TLKETFPVEA GLLRRSDRVL PKLLGQASLD APLLGRVGRT SRGLARLQLL ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQP LSRAAGRLSI HSLEAQSLRC LQPFCTQDTR DRPFQAQAQE SLDVLLRHPS GWWLVENEDR QTAWFPAPYL EEAAPGQGRE GGPSLGSSGP QFCASRAYES SRADELSVPA GARVRVLETS DRGWWLCRYG DRAGLLPAVL LRPEGLGALL SGTGFRGGDD PAGEARGFPE PSQATAPPPT VPTRPSPGAI QSRCCTVTRR ALERRPRRQG RPRGCVDSVP HPTTEQ |
| 分子量 | 41.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOXO1蛋白的3篇代表性参考文献的概括(注：以下内容为基于领域知识的示例性模拟，具体文献需通过学术数据库核实)：
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1. **文献名称**：*"Recombinant Human NOXO1 Expression and Its Role in NADPH Oxidase Assembly"*
**作者**：Takeya R, Sumimoto H
**摘要**：本研究利用大肠杆菌表达系统成功表达并纯化了重组人NOXO1蛋白，验证其与NOXA1的相互作用，证明NOXO1是NADPH氧化酶复合体(如NOX1)的关键组织者，并解析了其促进活性氧(ROS)生成的分子机制。
2. **文献名称**：*"Structural Insights into the SH3 Domain-Mediated Interaction of NOXO1 with NOXA1"*
**作者**：Lambeth JD, Cheng G
**摘要**：通过X射线晶体学揭示了重组人NOXO1蛋白的SH3结构域与NOXA1的特异性结合模式，阐明了二者在氧化酶复合体组装中的构象变化，为靶向NOXO1的抗氧化治疗提供结构基础。
3. **文献名称**：*"NOXO1-Dependent ROS Production in Colonic Epithelial Cells Promotes Inflammation"*
**作者**：Kawahara T, Rada B
**摘要**：在肠道炎症模型中，重组NOXO1蛋白的过表达显著增强上皮细胞ROS水平，激活促炎信号通路，提示NOXO1作为炎症性肠病(IBD)的潜在治疗靶点。
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**建议**：如需真实文献，可通过PubMed、Google Scholar等平台，以关键词“recombinant NOXO1”、“NOXO1 NADPH oxidase”检索最新研究，关注《Journal of Biological Chemistry》《Free Radical Biology and Medicine》等领域期刊。
NOXO1 (NADPH oxidase organizer 1) is a regulatory subunit of the NADPH oxidase complex, primarily involved in reactive oxygen species (ROS) generation. In humans, it functions as a critical component of the NOX1-dependent oxidase system, forming heterodimers with NOXA1 to activate NOX1. a membrane-bound enzyme pivotal for superoxide production. This interaction facilitates ROS signaling, which influences cellular processes like host defense, inflammation, and cell proliferation. Dysregulation of NOXO1 has been linked to pathologies, including cancer and chronic inflammatory diseases.
Recombinant human NOXO1 protein is engineered via molecular cloning and expression in bacterial or eukaryotic systems (e.g., E. coli or mammalian cells) to study its structure-function relationships and biological roles. Purified recombinant NOXO1 allows biochemical assays, crystallography, and interaction studies with NOX1/NOXA1. aiding mechanistic insights into NADPH oxidase regulation. Its production overcomes challenges in isolating native protein due to low endogenous expression. Research on recombinant NOXO1 also supports drug discovery targeting ROS-related disorders, offering potential therapeutic strategies by modulating oxidase activity. Current studies focus on its tissue-specific isoforms, post-translational modifications, and contributions to disease progression.
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